Deteksi Gen SHV pada Isolat Klinik Eschericia coli Penghasil Extended Spectrum Beta-Lactamases (ESBLs) dari Urin Pasien di RSUD Dr. Soetomo Surabaya

Yulianto Ade Prasetya

DOI: https://doi.org/10.23917/bioeksperimen.v4i2.6884

Abstract

Esherichia coli ESBLs bertanggungjawab terhadap terjadinya wabah infeksi nosokomial, peningkatan morbiditas dan mortalitas, serta peningkatan biaya kesehatan. Tujuan penelitian ini yakni untuk mendeteksi keberadaan gen SHV pada isolat klinik E.coli penghasil ESBLs dari urine pasien yang merupakan koleksi Laboratorium Mikrobiologi Klinik RSUD Dr. Soetomo Surabaya pada bulan Januari - Februari 2014. Jenis penelitian yang digunakan adalah observasional deskriptif dengan pendekatan molekul genetik. Metode yang digunakan untuk deteksi gen SHV menggunakan PCR (Polymerase Chain
Reaction) kemudian dielektroforesis dan divisualisasikan pada gel agarose 1.5%. Hasil penelitian menunjukkan bahwa dari tiga puluh isolat, sebanyak dua belas isolat (40%) positif mengandung gen SHV.

Keywords

ESBLs, Eschericia coli, RSUD Dr. Soetomo Surabaya, SHV

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References

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