POTENSI Azotobacter spp. SEBAGAI PENDEGRADASI LIPID DAN PROTEIN

Waritsatul Firdausi(1*), Enny Zulaika(2)

(1) Jurusan Biologi Institut Teknologi Sepuluh Nopember
(2) Jurusan Biologi Institut Teknologi Sepuluh Nopember
(*) Corresponding Author

Abstract

Azotobacter spp. merupakan salah satu genus bakteri nonsimbiotik yang berperan penting dalam bidang agrikultur terkait kemampuannya memfiksasi nitrogen. Penelitian ini bertujuan untuk mengetahui potensi isolat Azotobacter dari lahan Eco Urban Farming ITS dalam mendegradasi lipid dan protein. Kemampuan isolat dalam mendegradasi lipid dan protein ditentukan dengan pengukuran Indeks Biodegradasi (IB). Isolat yang digunakan adalah A1a, A1b, A2, A3, A5, A6, A7, A8, A9, dan A10. Semua isolat mampu mendegradasi lipid kecuali A5, dengan IB tertinggi pada isolat A1b (1,00). Semua isolat dapat mendegradasi protein kecuali A7, dengan IB tertinggi terdapat pada isolat A8 (3,00).

Keywords

Azotobacter spp.; lipid; protein; biodegradasi

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References

Breure, A.M. 2004. Soil Biodiversity: Measurements,Indicators, Threats and Soil Functions. Paper oral. Spain.

Gupta, P., L.S.B. Upadhyay, and R. Shrisvastava. 2011. Lipase CatalyzedTransesterification of Vegetable Oils by Lipolytic Bacteria. ResearchJournal of Microbiology. 6: 281-288.

Harley, and L.M. Prescott. 2002. Laboratory Exercise in Microbiology 5th Edition. USA: Mc Graw Hill.

Hastuti, U.S., P. Yakub, and H.N. Khasanah. 2013. Biodiversity of Indigenous Amylolytic and Cellulolytic Bacteria in Sago Waste Product at Susupu, North Moluccas. The Third Basic Science International Conference.

Kizilkaya, R. 2008. Nitrogen Fixation Capacity of Azotobacter spp. Strains Isolated from Soils in Different Ecosystems and Relationship Between Them and The Microbiological Properties of Soils. Journal of Environmental Biology. 30: 73-28.

Labrenz, S.R. 2014. Ester Hydrolysis of Polysorbate 80 in MAb Drug Product: Evidence in Support of The Hypothesized Risk After The Observation of Visible Particulate in MAb Formulations. J. Pharm. Sci. 103: 2268-77.

Pinto, G., S. Caira, M. Cuollo, S. Lilla, L. Chianese, and F. Addeo. 2012. Bioactive Casein Phosphopeptides in Dairy Products as Nutraceuticals for Functional Foods. J. Mol. Biol. 295: 7–16.

Suriadikarta, D.A., dan R.D.M. Simanungkalit. 2006. Pupuk Organik dan Pupuk Hayati. Jurnal Penelitian dan Pengembangan Pertanian. 1: 1-10.

Thakur, P.B. and C. Balomajumder. 2012. Biodegradation of O-Xylene by Azotobacter chroococcum. International Journal of Advanced Biotechnology and Research. 3: 502-508.

Widawati, S., Suliasih, H.J.D. Latupapua, dan A. Sugiharto. 2005. Biodiversity of Soil Microbes from Rhizosphere at Wamena Biological Garden (WBiG), Jayawijaya, Papua. Biodiversitas. 1: 6-11.

Zulaika, E., M. Shovitri and N.D. Kuswytasari.2012. Characterization and Identification Azotobacter From Kalimas Surabaya, Candidate for a Potential Biofertilizer and Mercury Bioreducer. Paper. Chulalongkorn University, Bangkok Thailand.

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