AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL DAN FRAKSI KULIT BATANG BELIMBING WULUH (Averrhoa bilimbi Linn.) TERHADAP BAKTERI Klebsiella pneumoniae DAN Staphylococcus epidermidis BESERTA BIOAUTOGRAFINYA

Dr. Muhtadi , MSi.(1*), Ria Ambarwati(2), Ratna Yuliani(3)

(1) 
(2) 
(3) 
(*) Corresponding Author

Abstract

Belimbing wuluh (Averrhoa bilimbi Linn.) is a tropical plant that has antibacterial properties. The purpose of this study was to test the antibacterial activity of bark Belimbing wuluh against Klebsiella pneumoniae and Staphylococcus epidermidis and their bioautography. Extraction methods used to research is method maceration with a solvent ethanol 96 %. Fractinations done by method partition liquid-liquid with a separating funnel. Test performed in this research covering identi� cation bacteria, the sensitivity bacteria, antibacterial activity, thin layer chromatography, bioautography. The result of antibacterial activity ethanol extract of disk diffusion method with concentrations 400 μg/disk, 800 μg/disk, 1600 μg/disk is 8±0,5; 10,34±0,58; 12,17±0,76 on Klebsiella pneumoniae, 10,17±0,29; 11±0; 11.5±0 on Staphylococcus epidermidis, n-hexane fraction with concentration 400 μg/disk, 800 μg/disk, 1600 μg/disk is 8,34±0,29; 9,34±0,29; 10,84±0,76 on Klebsialla pneumoniae, 8,5±0,5; 9,34±0,29; 10,67±0,29 on Staphylococcus epidermidis, ethyl acetate fraction with concentration 400 μg/disk, 800 μg/disk, 1600 μg/disk is 9,17±0,29; 10,34±0,29; 11,17±0,29 on Klebsiella pneumoniae and 9,5±0,5; 10,67±0,29; 12,67±1,26 on Staphylococcus epidermidis, ethanol-water fractions with concentration 400 μg/disk, 800 μg/ disk, 1600 μg/disk is 8,17±0,29; 9,17±0,29; 10±0 on Klebsiella pneumoniae, 9±0; 9,67±0,29; 10,34±0,29 on Staphylococcus epidermidis. The TLC show chemical compounds contained in the ethanol extract, n-heksan fraction, ethyl acetate fraction, and ethanol-water fraction is a compound of the saponins, alkaloids, � avonoids and phenolic. Bioautography showed that ethanol extracts, n-heksan faction, ethyl acetate fraction, and etanol-airfaction Belimbing wuluh (Averrhoa bilimbi Linn.) bark have not antibacterial activity because there is no clear area around on plate TLC.

Keywords: Belimbing wuluh (Averrhoa bilimbi Linn.), ethanol extract, fractination, antibacterial, bioautogra� .

Full Text:

PDF

References

Adisasmito, A. W. & Hadinegoro, S. R. S., 2004, Infeksi Bakteri Gram Negatif di ICU Anak: Epidemiologi, Manajemen Antibiotik dan Pencegahan, Sari Pediatri, 6 (1), 32-39.

Alam, G., Mu dah., Nasrum, M., Felix, K. & Umar., 2012, Skrining Komponen Kimia Dan Uji Aktivitas Mukolitik Ekstrak Rimpang Bangle (Zingiber purpureum Roxb.) Terhadap Mukosa Usus Sapi Secara In Vitro, Majalah Farmasi dan Farmakologi, 16 (3), 123 126.

Chowdhury, S. S., Uddin, G. M., Mumtahana, N.,Hossain, M. & Hasan, S. R. M., 2012, In Vitro Antioxidant and Cytotoxic Potential of Hydromethanolic Extract of Averrhoa bilimbi L. Fruits, International Journal of Pharmaceutical Sciences and Research, 3 (7), 2263-2268.

De Smet, P. A., 1997. The Role of Plant Derived Drugs and Herbal Medicines in Healthcare, Drugs, 54, 108.

Gibson, J. M., 1996, Mikrobiologi dan Patologi Modern untuk Perawat, diterjemahkan oleh Soma, P., 1, Jakarta, Penerbit Buku Kedokteran EGC.

Gomes, F., B. Leite, P. Teixeira & Oliveira.R., 2011, Strategies to control Staphylococcus epidermidis bio� lms, Journal of Science against microbial pathogens, 843-852

Jawetz, E, J. L., Adelberg, E. A., 1996, Mikrobiologi Kedokteran, Edisi XXII, Diterjemahkan oleh Bagian Mikrobiologi Fakultas Kedokteran Universitas Airlangga, Jakarta, Penerbit Salemba Medika.

Jawetz, E, J. L., Adelberg, E. A., 2001, Mikrobiologi Kedokteran, Edisi XXII, Diterjemahkan oleh Bagian Mikrobiologi Fakultas Kedokteran Universitas Airlangga, Jakarta, Penerbit Salemba Medika.

Jawetz, E, J.L., Adelberg, E. A., 2005, Mikrobiologi Kedokteran, Edisi XXII, Diterjemahkan oleh Bagian Mikrobiologi Fakultas Kedokteran Universitas Airlangga, Jakarta, Penerbit Salemba Medika.

Karon, B., Ibrahim, M., Mahmood, A., Huq, M., Chowdhury, U., Hossain, A., et al., 2011, Preliminary Antimicrobial, Cytotoxic and Chemical Investigations of Averrhoa bilimbi Linn. and Zizyphus mauritiana Lam. Bangladesh Pharmaceutical Journal, 14 (2), 127-131

Kumar, K., Gousia., Anupama, M., & Naveena, L., 2013, A Review On Phytochemical Constituents And Biological Assays of Averrhoa bilimbi L., International Journal of Pharmacy and Pharmaceutical Science Research. 3 (4), 136-139.

Harborne, J. B., 1996, Metode Fitokimia: Penuntun Cara Modern Menganalisis Tumbuhan, 2nd ed, diterjemahkan oleh Padmawinata K., Penerbit ITB, Bandung.

DOI: 10.23917/biomedika.v4i2.252

Article Metrics

Abstract view(s): 1034 time(s)
PDF: 1826 time(s)

Refbacks

  • There are currently no refbacks.