PENGARUH EKSTRAK ETHANOL PROPOLIS TERHADAP EKSPRESI PROTEIN Bcl2, CYCLIN D1 DAN INDUKSI APOPTOSIS PADA KULTUR SEL KANKER KOLON

Haryono Yuniarto, Sumardi Maryono, Bambang Purwanto

DOI: https://doi.org/10.23917/biomedika.v9i1.4343

Abstract

Kanker kolorektal menempati urutan kejadian kanker ketiga di seluruh dunia, dengan lebih dari 1 juta angka kejadian tiap tahunnya. Berbagai strategi terapi pengobatan kanker kolorektal tetapi relatif belum optimal. Oleh karena itu, terdapat kebutuhan mengembangkan terapi alternatif sebagai pendamping. Propolis menunjukkan aktivitas proapoptosis pada berbagai jenis sel kanker. Mengetahui pengaruh pemberian propolis yang berasal dari Kerjo, Karanganyar, Indonesia terhadap induksi proses apoptosis dan aktivitas antiproliferasi, terutama terkait dengan penekanan ekspresi protein Bcl 2 dan cyclin D1 pada kultur sel WiDr (cell line kanker kolon). Penelitian eksperimental laboratorik menggunakan post test with control group design. Penelitian dilakukan pada kultur sel WiDr (sel kanker kolon) dengan pemberian propolis. Pengamatan ekspresi protein Cyclin D1 dan Bcl2 dilakukan dengan metode imunositokimia, sedangkan pengamatan induksi apoptosis dilakukan dengan flowcytometry. Analisis statistik dengan uji Kruskal-Wallis, signifikan bila p <0,05. Rata-rata ekspresi Bcl2 pada kelima kelompok yaitu kontrol 83.40 ± 0.69 μg/ml, EEP 1/2 IC50 60.63 ± 0.40, EEP IC50 33.77 ± 1.08 μg/ml, EEP 2 IC50 24.28 ± 1.91 μg/ml, 5fluorouracil 12.74 ± 2.19 μg/ml. Terdapat perbedaan bermakna ekspresi Bcl2 antara kelompok uji dibandingkan kelompok kontrol (p < 0,001). Rata-rata ekspresi cyclin D1 pada kelima kelompok yaitu kontrol 83.77 ± 0.39 μg/ml, EEP 1/2 IC50 61.44 ± 0.41, EEP IC50 36.67 ± 1.18 μg/ml, EEP 2 IC50 24.50 ± 0.38 μg/ml, 5fluorouracil 13.42 ± 1.04μg/ml. Terdapat perbedaan bermakna ekspresi cyclin D1 antara kelompok uji dibandingkan kelompok kontrol (p < 0,001). Pemberian ekstrak etanol propolis mempunyai pengaruh menekan ekspresi Bcl2, cyclin D1, dan menginduksi apoptosis pada kultur sel kanker kolon (WiDr Cell Line).

 

Kata Kunci: Ekstrak Ethanol Propolis, Bcl2, cyclin D1, Sel WiDr

Full Text:

PDF

References

Alison, M.R. 2001. Cancer. In :Encyclopedia of Life Seciences. Nature Publishing Group. London. United Kingdom.

Departemen Kesehatan RI (Depkes RI). 2009. Profil Kesehatan Indonesia 2008. Departemen Kesehatan Republik Indonesia. Jakarta.

Diana S, Halina C, Maria H, Jacek N.2012. The anticancer activity of propolis. Folia hisyochemica et cytobiologica. Vol 50, No 1,pp 25-37

Ghobrial IM, Witzig TE, Adjei AA.2005, Targeting apoptosis pathways in cancer therapy. CA Cancer J Clin. 55(3):178-94.

Globocan. 2013. Cancer Fact Sheets for Colorectal Cancer.2013. Available from: URL: http://globocan.iarc.fr/Pages/fact_sheets_cancer.aspx

Ishiara M, Naoi K, Hashita M, Itoh Y, Suzui M. 2009. Growth inhibitory activity of ethanol extracts of Chinese and Brazilian propolis in four human colon carcinoma cell lines. Oncol Rep. 22: 349–354.

Kurniawati, A dan Tenggara, R. 2011. Pengaruh Asam Asetil Salisilat terhadap Penurunan Prevalensi Kanker Kolorektal. CDK. 186: 38(5): 350-352.

Kubina, R., Dzik, A.K., Dziedzic, A., Bielec, B., Wojtczka, R.D., Buldok, R.J., Wysznynska, M., et al. 2015. The ethanol extract of polish propolis exhibits anti-proliferative and/or pro-apoptotic effect on HCT 116 colon cancer and Me45 malignant melanoma cell in vitro conditions. AdvClinExp Med. 24(2): 203-212.

Marcucci MC, Ferreres F, Garc a-Viguera C, Bankova VS, De Castro SL, Dantas APet al, 2001. Phenolic compounds from Brazilian propolis with pharmacological activities. J Ethnopharmacol;74:p.105–12.

Motomura, M., Kwon, K.M., Suh, S.J. 2008. Propolis induces cell cycle arrest and apoptosis in human leukemic U937 cells through Bcl-2/Bax regulation. Environ ToxicolPharmacol. 26:61-67.

Nakajima, Y., Tsuruma, K., Shimazawa, M., Mishima.S., Hara, H. 2009. Comparison of Bee Products Based on Assays of Antioxidant Capacities. Nagaragawa Research Center. Department of Biofunctional Evaluation, Molecular Pharmacology, Gifu Pharmaceutical University, 5-6- 1 Mitahora-higashi, Gifu 502-8585. Japan. Published online by Journal BioMed Central Medicine, 1472- 6882/9:4

National Cancer Institute, 2009. What is cancer ?. National Institute of Health. United States.

Ottaiano, A., Franco, R., Aiello, T.A., Liguori, G., Tatangelo, F., Delrio, P., Nasti, G., et al. 2006. Overexpression of bothCXC chemokine receptor 4 and vascular endothelial growth factorproteins predicts early distant relapse in stage II-III colorectalcancer patients. Clin Cancer Res. 12: 2795-2803.

Paulino, N., Abreu, S.R.L., Machodo, G., Silveira, E. 2009. Scientific evidences to pharmacological anticancer action of Baccharisdracunculifolia Brazilian propolis. Rev PesqInov Farm. 1(1):15-26.

Robbins and Cotran, Pathologic Basis of Disease. 7th ed. WB Saunders Co. Philadelphia, 2005:309-13

Salatino A, Teixeira EW, Negri G, and Message D, 2005. Origin and Chemical Variation of Brazilian Propolis. Evid Based Complement Alternat Med 2(1): p.33–8

Siegel, R., Ma, J., Zou, Z., Jemal, A. 2014. Cancer statistics.CACancer J Clin.64: 9-29.

Syamsudin, Wiryowidagdo, S., Simanjuntak, P., Heffen, W.L. 2009. Chemical composition of propolis from different region in java and their cytotoxic activity. AJBB. 5(4): 180-183.

Torre, L.A., Bay, F., Siegel, R.L., Ferlay, J., Tieulent, J.L., Jemal, A. 2015. Global Cancer Statistic. Ca Cancer J Clin. 65: 87-108.

Vatansever, H.S., Sorkun, K. Gurhan, S.I.D., Ozdal-Kurt, F., Turkoz, E., Gencay, O., Salih, B. 2010. Propolis from Turkey induces apoptosis through activating caspases in human breast carsinoma cell lines. ActaHistochem. 112: 546-556.

Wang, X dan Zhang, Y. 2014. Targeting mTOR network in colorectal cancer therapy. World J Gastroenterol. 20(15): 4178-4188.

World Health Organization, International Agency for Research on Cancer, 2003. Human cancers by organ site; colorectal cancer. In: Stewart BW, Kleihues P, eds. World cancer report. Lyon, France: IARC Press, p:198–202.

Article Level Metrics

Refbacks

  • There are currently no refbacks.