PENGARUH SECRETOME SEL PUNCA MESENKIMAL TERHADAP EKSPRESI INTERLEUKIN-1β DAN CASPASE-1

Toumi Shiddiqi, Zainal Arifin Adnan, Arief Nurudhin

DOI: https://doi.org/10.23917/biomedika.v9i2.5840

Abstract

Systemic Lupus Erythematosus adalah penyakit inflamasi autoimun kronis dengan manifestasi klinis  beragam dan luas. Pemberian injeksi pristan intraperitoneal dapat menginduksi lupus pada mencit. IL-1β merupakan sitokin inflamasi yang berperan pada patogenesis SLE melalui jalur Caspase-1. Secretome sel punca mesenkimal mengandung Stanniocalcin-1 yang berperan sebagai antiinflamasi. Tujuan Penelitian adalah untuk mengetahui pengaruh secretome sel punca mesenkimal terhadap ekspresi IL-1β dan Caspase-1  pada mencit model lupus dengan induksi pristan. Penelitian eksperimental dengan metode post test only control group design, sampel 21 ekor mencit betina Mus Musculus galur Balb/C, dibagi 3 kelompok yaitu kelompok kontrol (injeksi intraperitoneal NaCl 0,9% 0,5 ml), kelompok pristan (injeksi pristan intraperitoneal 0,5 ml) dan kelompok pristan+secretome (injeksi intraperitoneal pristan 0,5 ml dan secretome 0,45 ml). Penelitian dilakukan selama 3 minggu, secretome diberikan pada akhir penelitian. Dinilai ekspresi IL-1β dan Caspase-1 secara imunohistokimia. Analisis  statistik menggunakan IBM SPSS 20 dengan uji ANOVA dilanjutkan uji post hoc LSD. P bermakna jika p<0,05. Hasil penelitian menunjukkan rata-rata ekspresi IL-1β pada ketiga kelompok yaitu kontrol (22,75±8,30); pristan (79,67±18,24); pristan+secretome (19,94±6,41), dengan kemaknaan p=0,000. Terdapat perbedaan bermakna ekspresi IL-1β antara kelompok kontrol vs pristan (-56,92±6,69; p=0,000), kelompok pristan vs pristan+secretome (61,31±6,69; p=0,000). Rata-rata ekspresi Caspase-1 pada ketiga kelompok yaitu kontrol(17,04±6,30); pristan (81,25±17,32); pristan+secretome (21,97±12,59), dengan kemaknaan p=0.000. Terdapat perbedaan bermakna ekspresi Caspase-1 antara kelompok kontrol vs pristan (-64,21±6,69; p=0,000) dan kelompok pristan vs pristan+secretome (59,28±6,69; p=0,000).Simpulan yang dapat diambil adalah  secretome sel punca mesenkimal menurunkan ekspresi IL-1β dan Caspase-1 pada mencit model lupus dengan induksi pristan.

Kata Kunci: Caspase-1, Interleukin-1β, Pristan, Secretome, Systemic Lupus Erythematosus.

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