Pengaruh Ekstrak Etanol Daun Murbei (Morus Alba L.) dengan Glibenklamid Terhadap Ekspresi Gen CYP3A4 pada Kultur Sel HepG2

Nuralih Nuralih(1), Churiyah Churiyah(2), Sabar Pambudi(3), Swasono R. Tamat(4), Okpri Meila(5*)

(1) Fakultas Farmasi, Universitas Pancasila Teknologi Farmasi dan Medika; BPPT Puspiptek Serpong
(2) Teknologi Farmasi dan Medika, BPPT Puspiptek Serpong
(3) Teknologi Farmasi dan Medika, BPPT Puspiptek Serpong
(4) Fakultas Farmasi, Universitas Pancasila
(5) Fakultas Farmasi Institut Sains dan Teknologi Nasional
(*) Corresponding Author

Abstract

Mulberry leaf is a traditional herb and predicted has ecdysteronecompound which act as antihyperglicemid. Glybenclamide is a synthetic medicine used to cure diabetes mellitus type 2. The leaf reported as competitive inhibitor of CYP3A4 enzyme, which metabolizingglibenclamide. However, in many cases, combination of herbs with synthesis drugs causes interaction if used at the same time. This research aimed to see interaction of ethanol mulberry leaf extract with glibenclamide through CYP3A4 gene expression in HepG2 cell culture.Sample of mulberry extract, glibenclamide, and combination both sample were tested into cell HepG2 culture. Then RNA were isolated and purification using real time PCR to see the gene CYP3A4 expression. As a result, mulberry extract acts as inhibitor enzyme CYP3A4, while glibenclamide is enzyme substrate.The combination of mulberry and glibenclamide showed increased of expression of CYP3A4 gene, means greater enzyme produced, and lower medicine on blood plasma.

Keywords

Diabetes mellitus; gene expression CYP3A4; glibenclamide; HepG2, mulberry

Full Text:

PDF

References

Ament PW, Bertolino JG, Liszewski JL. Clinical pharmacology: clinically significant drug interactions. Am Fam Physician. 2000; 61:1745-1754.

Gitawati R. 2008. Interaksi obat dan beberapa implikasinya. Media Litbang Kesehatan 18(4):175-184.

International Diabetes Federation. One adult in ten will have diabetes by 2030. 2011 [Diakses 18 Januari 2015] [http://www.idf.org/mediaevents/press-releases/2011/diabetes-atlas-8th-edition].

Adam I, Melva L, Arleni, SriWAJ, Franciscus DS. Influence of primaquine and ritonavir interaction on CYP3A4 mRNA expression in HepG2 cell culture. Medical Journal Indonesia. 2012; 2(1):3-7.

Teixeria L. Regular physical exercise training assists in preventing type 2 diabetes development: focus on its antioxidant and anti-inflammantory properties. Biomed Central Cardiovascular Diabetology. 2011; 10(2): 1–15.

Amit K, Pulok KM, Sankarsan S, Shiv B, SK Milan A and Subrata P. Possible herb-drug interaction of Morus alba L. α potential anti-diabetic plant from Indian Traditional Medicine. Indian Journal of Traditional Knowledge. 2015; 14(4): 626-631.

American Diabetes Association. Standards of medical care in diabetes. Diabetes care. 2012:11 – 13.

Katzung BG. Farmakologi dasar dan klinik. Ed VIII. Diterjemahkan oleh Dripa Sjabana. Surabaya: Bagian Farmakologi Fakultas Kedokteran Universitas Airlangga; 2002.

Yihai Wang, Limin Xiang, Chunhua Wang, Chao Tang, Xiangjiu He.Antidiabetic and antioxidant effects and phytochemicals of mulberry fruit (Morus alba L.) polyphenol enhanced extract. Plos One. 2013; 8(7): 1-10.

Duppala, V., Dash RP, Jivrajani MN, Thakur, 2Nirav M. Ravat SK, and Nivsarka M. Simultaneous Quantification of Glibenclamide, Simvastatin, and Quercetin by Using LC-UV Method and Its Application to Pharmacokinetic Study in Rats. Hindawi Publishing Corporation Chromatography Research International. 2013.

Amma NR. 2009. Efek hipoglikemik ekstrak daun murbei (Morusmulticaulis) terhadap kadar glukosa darah tikus dm. Tesis. Sekolah Pasca sarjana Institut Pertanian Bogor.

Sambrook J, Russel DW. Molecular cloning, a laboratory manual. Edisi III. New York: Cold Spring Harbor Laboratory Press; 2001: xxvi + 3.4 - 14.53 + 1.44.

Matsuda H, Kinoshita K, Sumida A, Koichi Takahashi K, Fukuena S, Fukuda T, et al. 2002. Taurine modulates induction of cytochrome P450 3A4 mRNA by rifampicin in the HepG2 cell line. Biochimica et Biophysica Acta 1593: 93–98.

Gerets HHJ, Tilmant K, Gerin B, Chanteux H, Depelchin BO, Dhalluin S, Atienzar FA. 2012. Characterization of primary human hepatocytes, HepG2 cells, and HepaRG cells at the mRNA level and CYP activity in response to inducers and their predictivity for the detection of human hepatotoxins. Cell Biol Toxicol 28:69-87, DOI 10.1007/s10565-011-9208-4.

DOI: 10.23917/pharmacon.v15i1.5766

Article Metrics

Abstract view(s): 1169 time(s)
PDF: 1691 time(s)

Refbacks

  • There are currently no refbacks.