Optimum Conditions for Extraction of Antibacterial Compounds from Citrus Aurantifolia Fruit Peel Waste

Rima Munawaroh

Abstract

Citrus fruit peel is a major waste in citrus fruit processing industry. The research on extraction active
compounds of Citrus aurantifolia (lime) fruit peel waste and antibacterial activity assay has been done. The
aim of research was to get optimum condition to extract their active compounds which have antibacterial
activity. The dried lime fruit peel was extracted by maceration method using ethanol 48%, 72%, and 96%.
The dried and fresh lime fruit peel were also extracted using ethyl acetate. Antibacterial assay was done by
diffusion agar against Staphylococcus aureus ATCC 25923 and Escherichia coli ATCC 25922. The result
showed that optimal condition to extract antibacterial compound using fresh sample with ethyl acetate as
solvent. The ethyl acetate extract of fresh sample was more active against S. aureus than E. coli.

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