Uji Aktivitas Antibakteri Ekstrak Etanol Bawang Dayak (Eleutherine palmifolia (L.) Merr) Terhadap Bakteri Staphylococcus aureus

Warsiti Warsiti, Sisca Dwikusuma Wardani, Ardea Achmad Ramadhan, Ratna Yuliani

DOI: https://doi.org/10.23917/pharmacon.v15i2.6526

Abstract

Staphylococcus aureus is an infectious bacteria with pus because of tissue damage. Infectious diseases caused by S. aureus include wound infections, ulcers, pimples, and impetigo. Dayak onions have the benefit of being antibacterial. This research aims to study the antibacterial activity of ethanolic extract of Dayak onions against  Staphylococcus aureus. The antibacterial study was carried out using the disk diffusion method. Identification of bacteria by Gram staining showed Gram-positive bacteria which can ferment mannitol with the mannitol test. A bacterial sensitivity test was conducted using three antibiotics i.e. cefoxitin 30μg, vancomycin 30μg, and linezolid 30μg. The bacteria was sensitive to all of the three antibiotics, with the inhibitory zone of 23.5 mm; 17 mm and 27.5 mm, respectively. Vancomycin 30μg was used as a positive control, resulted in average diameter of the inhibitory zone of 17.83  2.25 mm, whereas a control solvent DMSO (Dimethyl sulphoxide) did not produce zones. The ethanolic extract of Dayak onion in the concentration of 25%; 50%; 75% and 100% showed the average diameter of inhibitory zones of 8.17  0.29  mm; 10.67  1.44 mm; 10.33  1.53 mm; and 12.33  1.61  mm, respectively

Full Text:

PDF

References

Alves T.M.A., Helmut K. dan Carlos L.Z., 2003, Eleutherinone a Novel Fungitoxic Naphtoquinone from Eleutrine bulbosa (Iridiceae), Mem.Inst. Oswaldo Cruz. Rio de Janeiro, Vol 98(5): 709-712.

Departemen Kesehatan R.I., 2001, Inventaris Tanaman Obat Indonesia (I), Jilid 2, Departemen Kesehatan dan Kesejahteraan Sosial Republik Indonesia, Jakarta.

Febrinda A.E., 2013, Potensi Antioksidan dan Antidiabetik Ekstrak Air dan Etanol Umbi Bawang Dayak (Eleutherine palmifolia) Secara In Vitro dan In Vitro [Disersasi], Sekolah Pascasarjana IPB, Bogor.

Galingging R.Y., 2009, Bawang Dayak (Eleutherine palmifolia (L.) Merr) Sebagai Tanaman Obat Multifungsi, Warta Penelitian dan Pengembangan, Vol 15, No 3, Hal 2-4.

Gandjar I.G., dan Rohman A., 2007, Kimia Farmasi Analisis, Pustaka Pelajar, Yogyakarta.

Giardani R.N., 2017, Perbandingan Daya Hambat Ekstrak Etanol Bawang Dayak (Eleutherine Palmifolia L.) Dengan Bawang Merah (Allium Ascalonicum L.) Terhadap Bakteri Staphylococcus Aureus, Karya Tulis Ilmiah, Stikes Nasional, Surakarta.

Hidayah A., Mulkiya K., dan Purwanti L., 2015, Uji Efektivitas Antioksidan Bawang Dayak (Eleutherine bulbosa L.), Laporan Penelitian, UNISBA, Jakarta.

Jawetz M.G.E., and Adelberg C.A., 2001, Mikrobiologi Kedokteran Edisi I, Bagian Mikrobiologi Fakultas Kedokteran Universitas Airlangga, Surabaya.

Jawetz M.G.E. and Adelberg C.A. 2008. Mikrobiologi Kedokteran. Jakarta: Penerbit Buku Kedokteran EGC.

Kusuma S.A.F., 2009, Uji Biokimia Bakteri, Karya Ilmiah, Universitas Padjajaran, Bandung.

Nuur A.P.E., dan Haryoto, 2018, Uji Aktivitas Antikanker Kombinasi Eksrak Etanol Umbi Bawang Dayak (Eleutherine Amricana Merr.) dan Biji Sirsak (Annona Muricata) Dengan Metroteksat Terhadap Sel T47d, Skripsi, Univesitas Muhammadiyah Surakarta, Surakarta.

Pratiwi A.T., 2008, Mikrobiologi Farmasi, Penerbit Erlangga, Jakarta.

Puspadewi R., Adirestuti P., dan Menawati, R., 2013, Khasiat Umbi Bawang Dayak (Eleutherine palmifolia (L.) Merr) Sebagai Herbal Antimikroba Kulit, Kartika Jurnal Ilmiah Farmasi, 1 (1), 31-37.

Ryan K.J., Champoux, Falkow, Plonde, Drew, Neidhardt, and Roy, 1994, Medical Microbiology An Introduction to Infectious Diseases 3 rd ed, Appleton&Lange, Connecticut.

Sa'adah, H., 2017, Pengaruh Metode Ekstraksi Terhadap Kadar Flavonoid Ekstrak Etanol Umbi Bawang Dayak (Eleutherine palifollia (L.) Merr) Dengan Metode Spektrofotometri, Journal, Akademi Farmasi, Samarinda.

Article Metrics

Abstract view(s): 852 time(s)
PDF: 1541 time(s)

Refbacks

  • There are currently no refbacks.