DAYA ANTIBAKTERI EKSTRAK ETANOL DAUN SENGGANI (Melastoma affine D. Don)

Ika Trisharyanti Dian Kusumowati(1*), Rosita Melannisa(2), Angga Prasetyawan(3)

(1) 
(2) 
(3) 
(*) Corresponding Author

Abstract

Melastoma affine D. Don had some activities such as anthelmintic, antibacteria, antiinfiammation, antifungal, and antitumor. The aims of this research was determine antibacteria activity of ethanolic extract of Melastoma affine D. Don. The antimicrobial activity was tested by solid dilution method to get Minimum Inhibition Concentration (MIC). The compounds in Melastoma affine D. Don was analyzed by tube test method and Thin Layer Chromatography (TLC) with chloroform : methanol : formic acid (8,5:1,5:0,5) as mobile phase and silica gel GF254 as stationary phase. The result showed ethanolic extract of Melastoma affine D. Don contains alkaloid, polyphenol, fiavonoid, saponin, and essential oil. The MIC of Senggani against Staphylococcus aureus was 2% and 3% against Escherichia coli and the extract could not inhibit Staphylococcus aureus and Escherichia coli multiresistant until concentration 7% extract ethanol.


Keywords: Melastoma affine D. Don, Staphylococcus aureus, Escherichia coli

Full Text:

PDF

References

Anonim. 1989. Materia Medika Indonesia. Jilid V. Departemen Kesehatan Republik Indonesia. Jakarta. 549-553

Dalimartha, S. 2000. Atlas Tumbuhan Obat Indonesia. Jilid I. Trubus Argo Widya. Jakarta. 68-69.

Hertiani T., Palupi, I.S., Sanliferianti, & Nurwindasari, H.D. 2003. Uji Potensi Antimikroba terhadap S. aureus, E. coli, Shigella dysentriae, dan Candida albicans dari Beberapa Tanaman Obat Tradisional untuk Penyakit Infeksi. Pharmacon. Vol. 4 No. 2. UMS. Surakarta.

Jawetz, E., Melnick, J.L., & Adelberg, E.A. 2005. Mikrobiologi Kedokteran. diterjemahkan oleh Mudihardi, E., Kuntaman, Wasito, E.B., Mertaniasih, N.M., Harsono, S., Alimsardjono, L. Edisi XXII. Penerbit Salemba Medika. Jakarta. 327-335. 362- 363.

Robbers, S.E., Speedie, M.K., Tyler, V.E. 1996. Pharmacognosy and Pharmacobiotechnology. A. Waverly Company. USA. 139-140.

Robinson, T. 1995. Kandungan Organik Tumbuhan Tinggi. Edisi VI. diterjemahkan oleh Padmawinata, K. Institut Pertanian Bandung. Bandung.

Titi, N.W., Rusmiati, D., Farida, Y. 2007. Aktivitas Antibakteri Ekstrak Dan Fraksi Ekstrak Daun Senggani (Melastoma malabathricum Linn.) Terhadap Staphylococcus aureus, Escherichia coli, Dan Bacillus cereus Dengan Metode Difusi Agar. Farmaka. 5 (3).

Tjay dan Raharja. 2002. Obat-obat Penting: Khasiat, penggunaan dan Efek-efek Samping. Departemen Kesehatan Republik Indonesia. Jakarta.

Wagner, H. & Bladt, S. 1996. Plant Drug Analysis A Thin Layer Chromatography Atlas. Second Edition. Springer German.

DOI: 10.23917/biomedika.v6i2.278

Article Metrics

Abstract view(s): 2524 time(s)
PDF: 4889 time(s)

Refbacks

  • There are currently no refbacks.