Karakterisasi Isolat Bakteri Penghasil Kitinase Dan Glukanase Serta Uji Efektifitasnya Terhadap Jamur Colletotrichum sp

yadi - suryadi(1*), Dwi N susilowati(2), I Made - Samudra(3)

(1) BB Biogen
(2) Lab Microbiology BB Biogen
(3) Lab Biokimia
(*) Corresponding Author

Abstract

Shallots as a horticultural commodity is quite important in Indonesia. Fungi anthracnose disease caused by fungus Colletotrichum sp. is one of the pathogens  causing rot on shallot plants. Therefore, an alternative control that is environmentally friendly is a necessity. The aim of the study was to screen the best potential isolates producing chitinase and glucanase of shallot- rhizosphere from Brebes, and further used to inhibit fungal pathogens. The results showed that it was obtained 8 isolates have suppression activity against Colletotrichum sp. which varied in their inhibition. The highest inhibition was produced by isolate UBS 3 (30.56%), whilst one isolate (TK 3) did not have the ability to inhibit Colletotrichum sp.

Keywords

shallot, rhizosfera, chitinase, glucanase, Colletotrichum sp

References

Badan Pusat Statistik (BPS). 2013. Data produksi Tanaman sayuran 2012. BPS, Jakarta

Fauziah, dan N., Herdyastuti. 2013. Uji aktivitas bakteri kitinolitik dari tambak udang di lamongan dan sidoarjo. UNESA J.Chemistry. 2(1):36-39.

Ferrari, A.R., Y. Gaber., and Fraaije. 2014. A fast, sensitive and easy colorimetric assay for chitinase and cellulase activity detection. Biotechnol. for Biofuels. 7(37): 1-8.

Haedar, N., H. Natsir., Fahruddin., dan W., Aryanti. 2017. Produksi dan karakterisasi enzim kitinase dari bakteri kitinolitik asal kerang anadara granosa. J. Ilmu Alam dan Lingkungan. 8 (15) : 14 – 21.

Hamid, R., M.A. Khan., M. Ahmad., M.M. Ahmad., M.Z. Abidin., J. Musarrat., and S., Javed, 2013. Chitinase: an update. J. Pharmacy and BioAllied Sciences. 5(1): 21-29.

Hardestyariki, D., B. Yudono., dan Munawar. 2013. Eksplorasi bakteri hidrokarbonoklastik dari rhizosfer di lahan tambang minyak rakyat, kecamatan babat toman, Sumatera Selatan. J. Penelitian Sains. 16(3): 78-85.

Irfan, M. 2013. Respon bawang merah (Allium ascalonicum L) terhadap zat pengatur tumbuh dan unsur hara. J. Agroteknologi. 3(2): 35-40.

Manzila, I., T.P. Priyatno., M.F. Fathin., L. Ambarsari., Y. Suryadi., I.M. Samudera., dan D.N..Susilowati. 2015. Karakterisasi β-1,3-1,4-glukanase bakteri endofitik Burkholderia cepacia isolat E76 asal tanaman padi. Berita Biologi. 14(2): 143-153.

Pelczar, M.J., and Chan. 2007. Dasar-Dasar Mikrobiologi. Ratna Siri Hadioetomo, Teja Imas, S. Sutarmi Tjitrosomo, Sri Lestari Angka, Penerjemah. Jakarta (ID): UI Press. Terjemahan dari: Elements of Microbiology.

Pratiwi, R.S., T.E. Susanto., Y.A.K. Wardani., dan A., Sutrisno. 2015. Enzim kitinase dan aplikasi di bidang industri: kajian pustaka. J. Pangan dan Agroindustri.3(3): 878-88.

Prochnow, A.M., M. Clauson., J. Hong., and A.B., Murphy. 2016. Gram positive and gram negative bacteria differ in their sensitivity to cold plasma. Scientific Repost 6(3): 1-11.

Rathore, A.S., and R.D.. Gupta. 2015. Chitinases from bacteria to human: properties, applications, and future perspectives. Enzyme Research. 1(2): 1-8.

Salim, M.A. 2012. Pengaruh antraknosa (Colletotrichum capsici dan Colletotrichum acutatum ) terhadap respons ketahanan delapan belas genotipe buah cabai merah (Capsicum annuum L). J. Sains. 6(2): 182-187.

Senol, M., H. Nadaroglu., N. Dikbas., and R., Kotan. 2014. Purification of chitinase enzymes from Bacillus subtilis bacteria tv-125, investigation of kinetic properties and antifungal activity against Fusarium culmorum. Annals of Clinical Microbiology and Antimicrobials. 13(35): 1-7.

Setia, I., dan Suharjono. 2015. Diversitas dan Uji Potensi Bakteri Kitinolitik dari Limbah Udang. J. Biotropika. 3(2): 95-98.

Suryadi, Y., T.P. Priyatno., I.M. Samudera., D.N. Susilowati., N. Lawati., dan E, Kustaman. 2013. Pemurnian parsial dan karakterisasi kitinase asal jamur entomopatogen Beauveria bassiana isolat BB200109. J. AgroBiogen. 9(2): 77-84.

Suryadi, Y., D.N. Susilowati, P. Lestari, T.P. Priyatno, I.M. Samudra, N. Hikmawati dan N.R. Mubarik. 2014. Characterization of bacterial isolates producing chitinase and glucanase for biocontrol of plant fungal pathogens Journal of Agricultural Technology Vol. 10(4): 983-999

Yang, D.C., M. Kris., and N.R., Salama. 2016. Staying in shape: the impact of cell shape on bacterial survival in diverse environments. Microbiology and Molecular Biology Reviews. 80(1): 187-203.

Yusra., F. Azima., Novelina., dan Periadnadi. 2014. Isolasi dan identifikasi mikroflora indigenous dalam budu. Agritech. 34(3): 316-321.

DOI: 10.23917/bioeksperimen.v7i2.11197

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