EKOGENOTOKSISITAS LIMBAH CAIR BATIK DAN EFEK ANTIMUTAGENIK Lemna minor TERHADAP ERITROSIT IKAN NILA (Oreochromis niloticus)

Erma Musbita Tyastuti(1*), Okid Parama Astirin(2), Sunarto Sunarto(3)

(1) Universitas Sebelas Maret
(2) Universitas Sebelas Maret
(3) Universitas Sebelas Maret
(*) Corresponding Author

Abstract

Limbah cair batik di Solo sebagian besar dibuang langsung ke perairan tanpa diolah terlebih dahulu dan menyebabkan pencemaran air. Kandungan logam berat di dalam limbah cair batik dapat memicu efek genotoksik seperti pembentukan mikronukleus. Lemna minor berpotensi sebagai antimutagen dan mencegah pembentukan mikronukleus karena mengandung senyawa aktif seperti karoten dan asam amino. Penelitian ini bertujuan untuk mengetahui ekogenotoksisitas limbah cair batik dan efek antimutagenik Lemna minor terhadap eritrosit ikan nila. Penelitian dilakukan di Laboratorium Biologi UMS dengan pemaparan limbah cair batik 0ppm/L, 2500 ppm/L, 5000 ppm/L dan 7500 ppm/L terhadap 2 kelompok ikan nila dengan diet pelet dan Lemna minor. Hasil penelitian menunjukkan bahwa paparan limbah cair batik memicu pembentukan mikronukleus dengan frekwensi tertinggi pada konsentrasi paparan 7500 ppm/L. Lemna minor juga terbukti memiliki potensi antimutagenik karena mampu menekan frekwensi mikronukleus lebih rendah dibandingkan diet pelet.

Keywords

limbah cair batik, ekogenotoksisitas; mikornukleus; Lemna minor; antimutagen

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References

Akpoilih, B. U. 2012. “Fish Ecogenotoxicology : An Emerging Scinece, An Emerging Tool for Environmental Monitoring and Risk Assessment”. G.J.B.B. 1 (2) : 141-151. ISSN : 2278-9103

Ali, M., Sukirno, Tamzil, M. H. and Ichsan, M. 2014. “Meat Traits of Msucovy Ducks Fed on Phytonutrition Meal”. Int. J. Poult. Sci. 13 (4) : 204-207. ISSN : 1682-8356

Anderson, G. 2007. “Mechanism of Iron Loading and Toxicity”. Am. J. Hematol. 82 : 1128-1131. DOI : 10.1002/ajh.21075

Arunachalan, K.D., Annamalai, S.K. and Kuruva, J. K. 2013. “In Vivo Evaluation of Hexavalent Chromium Induced DNA Damage by Alkaline Comet Assay and Oxidative Stress in Catla catla”. AJES 9 (6) : 470-482. DOI : 10.3844/ajessp.2013.470.482

Aryani, Y., Sunarto dan Widiyani, T. 2004. “Toksisitas Akut Limbah Cair Batik CV. Giyant Santoso Surakarta dan Efek Sublethalnya Terhadap Struktur Mikroanatomi Branchia dan Hepar Ikan nila (Oreochromis niloticus)”. BioSMART. 6 (2) : 147-153. ISSN : 1412-033x

Ayoola, S. O. and Akaeze, c. O. 2012. “Genotoxic Evaluation and Toxicity of Spent Engine Oil on Clarias gariepinus”. Res. J. Environ. Toxicol. 6 (4) : 133-141. ISSN : 1819-3420. DOI : 10.3923/rjet.2012.133.141.

Azeez, N. M. and Sabbar, A. A. 2012. “Efficiency of Duckweed (Lemna minor L.) in Phytotreatment of Wastewater Pollutants from Basrah Oil Refinery”. J. Appl. Phytotech. Environ. Sanit. 1 (4) : 163-172. ISSN : 2088-6586

Bhattacharya, Sanjib. 2011. “Natural Antimutagens : A Review”. Res. J. Med. Plant. 5 (2) : 116-126. ISSN : 1819-3455. DOI : 10.3923/rjmp.2011.116.126

Bucker, A. and da Conceicao, M. B. 2012. “Genotoxicity evaluation of tilapia (Oreochromis niloticus) exposed to waters from two sites of Itajai-Acu River (SC, Brazil)”. J. Braz. Soc. Ecotoxicol. 7 (2) : 51-56. DOI : 10.5132/jbse.2012.02.008

Cadahia, B. P., Laffon, B., Porta, M., Lafuente, A., Cabaleiro, T., Lopez, T., Caride, A., Pumarega, J., Romero, A., Pasaro, E. and Mendez, J. 2008. “Relationship Between Blood Concentration and Endocrine Parameters Among Subjects Involved in Cleaning Coastal Areas Affected by The ‘Prestige’ Tanker Oil Spill”. Chemosphere. 71 : 447-455. DOI :10.1016/j.chemosphere.2007.10.053

Da Rocha, C. A. M. 2011. “The micronucleus Test in Erythrocytes of Amphibian Larvae as Tool for Xenobiotic Exposure Risk Assessment : A Brief Review and An Example Using Lithobates catesbieanus Exposed to Copper Sulphate”. Middle-East. J. Sci. Res. 8 (1) : 23-29. ISSN : 1990-9233

Dubey, A. and Tripathi, N.K. 2014. “Evaluation of Time, Dose, and Tissue Dependent Genotoxic Effect of Cadmium Chloride in a Freshwater Fish, Channa punctatus (Pisces : Family : Channidae)”. IJRSR 5 (5) : 934-939. ISSN : 0976-3031

Dziaman, T., Jurgowiak, M. and Olinski, R. 2011. “Association between body iron stores and level of oxidatively modified DNA bases”. JBCBB 92 (2) : 159-165

Flora, S. J. S., Mittal, M. and Mehta, A. 2008. “Heavy metal induced oxidative stress and its possible reversal by chelation therapy”. Indian. J. Med. Res. 128 : 501-523

Gulcin, I., Kurecci, E., Akkenik, E., Topal, F. and Hisar, O. 2010. “Antioxidant, Antibacterial and Anticandidal Activities of Aquatic Plant : Duckweed (Lemna minor L. Lemnaceae). Turk. J. Biol. 34 : 175-188

Hartati, I., Riwayati, I. dan Kurniasari, L. 2011. “Potensi Xanthate Pulpa Kopi Sebagai Adsorben Pada Pemisahan Ion Timbal dari Limbah Industri Batik”. Momentum. 7 (2) : 25-30

Horta, R.N., Kahl, V. F. S., Sarmento, M. S., Nunes, M. F. S., Porto, C. R. M., de Andrade, V. M., Ferraz, A. B. F. and Da Silva, J. 2016. “Protective effects of acerola juice on genotoxicity induced by iron in vivo”. GMB 39 (1) : 122-128. DOI : 10.1590/1678-4685-GMB-2015-0517

Jaishankar, M., Tseten, T., Anbalagan, N., Mathew, B. B. and Beeregowda, K. 2014. “Toxicity, mechanism and health effects of some heavy metals”. Interdiscip. Toxicol. 7 (2) : 60-72. DOI : 10.2478/intox-2014-0009

Khellaf, N. and Zerdaoui, M. 2010. “Growth, Photosynthesis and respiratory Response to Copper in Lemna minor : A Potential Use of Duckweed in Biomonitoring”. Iran. J. Environ. Health. Sci. Eng. 7 (2) : 299-306

Kousar, S and Javed, M. 2015. “Studies on Induction of Nuclear Abnormalities in Peripheral Blood Erythrocytes of Fish Exposed to Copper”. Turk. J. Fish. Aquat. Sci. 15 : 879-886. ISSN : 1303-2712. DOI : 10.4194/1303-2712-V15_4_11

Kumar, R., Nagpure, N.S., Kushwaha, B., Srivastava, S. K. and Lakra, W. S. 2010. “Investigation of the genotoxicity of malathion to freshwater teleost fish Channa punctatus (Bloch) using the micronucleus test and commet assay”. Arch. Environm. Contam. Toxicol. 58 : 123-130. DOI : 10.1007/s00244-009-9354-3

Lin, C. C., Wu, M. L., Yang, C. C., Ger, J., Tsai, W. J. and Deng, J. F. 2009. “Acute Severe Chromium Poisoning After Dermal Exposure to Hexavalent Chromium”. J. Chin. Med. Assoc. 72 (4) : 219-221. DOI : 10.1016/s1726-4901 (09) 70059-0

Luzhna, L., Kathiria, B. and Kovalchuk, O. 2012. “Micronuclei in Genotoxicity Assessment : From Genetics to Epigenetics and Beyond”. Frontiers in Genetics. 4 Article 131. DOI : 10.3389/fgene.2013.00131

Marara, A. 2012. “Plant Response to Heavy Metal Toxicity” in A. Furim (ed). Plants and Heavy Metals. Springerbriefs in BioMetals. DOI : 10.1007/978-94-007-4441-7_2

Martin, S.and Griswold, W. 2009. “Human Health Effects of Heavy Metals”. Environmental Science and Technology Briefs for Citizens. 15th Ed. CHSR. KSU. Manhattan. New York

Mauro, M. O., Pesarini, J. R., Ishii, P. L., daSilva, A. F. and Oliveira, R. J. 2010. “Chemopreventive Activity of Phenylalanine Againts Damage Mutagenic Prompted by the Acute Administration of Cyclophosphamide in Pregnant and Non-Pregnant Mice Using the Micronucleus Test”. Braz. J. Pharmacogn. 20 (3) : 334-339

Mir, M. I., Khan, S., Bhat, S. A., Reshi, A. A., Shah, F. A., Balki, M. H. and Manzoor, R.2014. “Scenario of Genotoxicity in Fishes and Its Impact on Fish Industry”. IOSR-JESTFT. 8 (6) : 2319-2402. E-ISSN : 2319-2402

Mohod, C. V. and Dhote, J. 2013. “Review of Heavy Metals in Dringking Water and Their Effect on Human Health”. IJIRSET. 2 (7) : 2992-2996

Nagarathna, P. K. M., wesley, M. J., Reddy, P. S. and Renna, K. 2013. “Review on Genotoxicity, Its Molecular Mechanism and Prevention”. Int. J. Pharm. Sci. Rev. Res. 22 (1) : 236-243. ISSN : 0976-044x

Okocha, R. O. and Adedeji, O. B. 2012. “Overview of Copper Toxicity to Aquatic Life”. Rep. Opinion. 4 (8) : 57-67. ISSN : 1553-9873

Olaniyi, C. O. and Oladunjaye, I. O. 2012. “Replacement Value of duckweed (Lemna minor) in Nile tilapia (Oreochromis niloticus) Diet”. Transnat.J. Sci. Tech. 2 (9) : 54-61

Ozkan, F., Gunduz, S. G., Berkoz, M. and Ozlner Hunt, A. 2011. “Induction of micronuclei and other nuclear abnormalities in peripheral erythrocytes of Nile tilapia, Oreochromis niloticus, following exposure to sublethal cadmium dose”. Turk. J. Zool. 35 (4) : 585-592. DOI : 10.3906/zoo-0907-77

Paczkowska, M., Kozlowska, M. and Golinski, P. 2007. “Oxidative Stress Enzyme Activity in Lemna minor L. Exposed to Cadmium and Lead”. ACTA BIOLOGICA CRACOVIENSIA Series Botanica. 49 (2) : 33-37. PL ISSN : 0001-5296

Papanikolaou, G. and Pantopoulos, K. 2005. “Iron metabolism and toxicity”. Toxicol. Appl. Pharmacol. 202 : 199-211. DOI : 10.1016/jtaap.2004.06.021

Putra, D. E., Astuti, F. P. dan Suharyadi, E. 2014. “Studi Penurunan Kadar Logam Besi (Fe) pada Limbah Batik dengan Sistem Purifikasi Menggunakan Absorben Nanoparrtikel Magnetic (Fe3O4)”. Prosiding Pertemuan Ilmiah XXVIII HFI Jateng dan DIY : 250-252. ISSN : 0853-0823

Radic, S., Stipanicev, D., Cvjetko, P., Mikelic, I. L., Rajcic, M. M., Sirac, S., Kozlina, B. P. and Pavlica, M. 2009. “Ecotoxicological Assessment of Industrial Effluent Using Duckweed (Lemna minor L.). as A Test Organism”. Ecotoxicology. DOI 10.1007/s10646-009-0408-0

Rasal, K., Rasal, A. and Makwana, N. 2011. “Micronucleus test as a cytogenetic marker for evaluation of genotoxicity in fish, Labeo rohita”. Asian J. Animal. Sci. 6 (1) : 32-34

Roy, M. K., Kuwabara, Y., Hara, K., Watanabe, Y. and Tamai, Y. 2002. “Antimutagenic Effects of Amino Acids on the Mutagenicity of N-methyl-N-nitro-N-nitrosoguadinine (MNNG)”. Biosc. Biotechnol. Biochem. 66 (6) : 1400-1402. DOI : 10.1271/666.66.1400

Sasongko, Dwi P. 2010. “Identifikasi Unsur dan Kadar Logam Berat pada Limbah Pewarna Batik dengan Metode Analisis Pengaktifan Neutron”. TELAAH. 27 : 22-27

Shadreck, M. and Mugadza, T. 2013. “Chromium, an essential nutrient and pollutant : A Review”. AJPAC 7 (9) : 310-317. DOI : 10.5897/AJPAC 2013.0517

Shugart, L. and Theodorakis, C. 1998. “New Trends in Biological Monitoring : Application of Biomarkers to Genetic Ecotoxicology”. Biotherapy. 11 : 119-127

Sloczynska, K., Powraznik, B., Pekala, E. and Waszkielewicz. 2014. “Antimutagenic Compounds and Their Possible Mechanism of Action”. J. Appl. Genetics. 55 : 273-285. DOI : 10.1007/513355-014-0198-9

Srivastava, P. and Singh, A. 2015. “Evidence of Micronuclei in Fish Blood as A Biomarker of Genotoxicity due to Surface Run of Agricultural Fungicide (Propiconazole)”. J Toxicol. Environ. Health. Sci. 7 (1) : 4-8. Article Number : 845D77C50247. ISSN : 2006-9820. DOI : 10.5897/JTEHS2015.0325

Srividya, A. R., Dhanabal, S. P. and Vishnuvarthan, V. J. 2012. “Mutagenicity/Antimutagenicity of Plant Extracts Used in Traditional Medicine : A Review”. WJPR. 2 (1) : 236-259. ISSN : 2277-7105

Stephenson, A. P., Mazu, T.K., Miles, J. S., Freeman, M. D., Reams, R. R. and Flores-Rozas, H. 2013. “Defects in Base Excision Repair Sensitize Cells to Manganese in S. cerevisiae”. BioMed. Res. Int. Article ID 295635. DOI : 10.1155/2013/295635

Subki, N. S., Hashim, R. and Muslim, N. Z. M. 2014. “Heavy Metals Analysis of Batik Industry Wastewater, Plant and Soil Samples : A Comparison Study of FAAS and HACH Colorimeter Analytical Capabilities” in A. Z. Aris et al., (Eds) From Sources to Solution pages 285-289. Springer Science and Business Media. Singapore

Thakur, B. R., Singh, R. K., Handa, A. K. and Rao, M. A. 1997. “Chemistry and uses of pectin – A Review”. Critical Rev. In Food Sci. and Nutri. 37 (1) : 47-73. DOI : 10.1080/10408399709527767

Toyokuni, S. 2009. “Role of Iron in Carcinogenesis : Cancer as a ferrotoxic disease”. Cancer Sci. 100 (1) : 9-16. DOI : 10.1111/j.1349-7006.2008.01001.x

Yilmaz, E., Akyurt, I. and Gunal, G. 2004. “Use of Duckweed. Lemna minor, as A Protein Feedstuff in Practical Diets for Common Carp, Cyprinus carpio, Fry”. Turk. J. Fish. Aquat. Sci. 4 : 105-109

Zhao, Z. Y., Liang, L., Fan, X. Q., Yu, Z. H., Hotchkiss, A. T., Wilk, B. J. and Eliaz, I. 2008. “The Role of Modified Citrus Pectin As An Effective Chelator of Lead in Children Hospitalized with Toxic Lead Levels”. Altern. Ther. Health. Med. 14 (4) : 34-38

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