VITAMIN E PREVENTS OXIDATIVE STRESS AND INFLAMMATION CONDITIONS IN PERIODONTITIS WISTAR RATS

Maiyani Lestari(1), Rauza Sukma Rita(2*), Isnindiah Koerniati(3)

(1) Master Program in Biomedical Sciences, Medical Faculty, Universitas Andalas, Indonesia
(2) Biochemistry Department, Medical Faculty, Universitas Andalas, Indonesia
(3) Dental and Oral Department, Medical Faculty, Universitas Andalas, Indonesia
(*) Corresponding Author

Abstract

ABSTRAK

Periodontitis terutama disebabkan bakteri patogen periodontal yang dapat memicu pembentukan radikal bebas yang berlebihan, menyebabkan stres oksidatif sehingga terjadi kerusakan jaringan. Hal ini mempunyai pengaruh bermakna terhadap peningkatan produksi Reactive Oxygen Species (ROS) dalam tubuh dan kerusakan sel pada jaringan alveolar gigi. Penelitian ini bertujuan untuk mengetahui pengaruh vitamin E dalam mengatasi stres oksidatif pada tikus putih strain Wistar yang mengalami periodontitis. Penelitian ini merupakan penelitian eksperimental dengan post test only control group design. Tiga puluh ekor tikus dikelompokan menjadi 5 kelompok yaitu kontrol negatif (tanpa perlakuan), kontrol positif (diberi silk ligature 14 hari) dan perlakuan 1, 2, dan 3 (diberi silk ligature selama 14 hari, dilanjutkan dengan pemberian vitamin E dengan dosis 20, 40, dan 60 IU selama 10 hari). Pada hari terakhir penelitian dilakukan pengambilan darah untuk pemeriksaan kadar Malondialdehyde (MDA) dan penilaian inflamasi gingiva melalui skor indeks gingiva. Hasil penelitian menunjukkan bahwa rerata kadar MDA pada kelompok kontrol positif yang diberikan silk ligature saja mempunyai kadar MDA tertinggi dibandingkan dengan kelompok kontrol negatif dan semua kelompok perlakuan. Pemberian vitamin E mampu menurunkan kadar MDA serum secara bermakna pada semua kelompok perlakuan (p < 0,05). Vitamin E juga mampu menurunkan skor indeks gingiva dibandingkan dengan kelompok kontrol positif pada semua kelompok. Simpulan: Pemberian vitamin E mampu mengatasi stres oksidatif dan inflamasi pada periodontitis yang ditandai dengan penurunan kadar MDA dan skor indeks gingiva.

Kata Kunci: Vitamin E, Stress Oksidatif, Inflamasi, Periodontitis

ABSTRACT

Periodontitis is mainly caused by periodontal pathogenic bacteria, which can trigger excessive free radical formation, causing oxidative stress and tissue damage. This has a significant effect on increasing the production of Reactive Oxygen Species (ROS) in the body and cell damage in the alveolar tissue of the teeth. This study aimed to determine the effect of vitamin E in overcoming oxidative stress in Wistar strain white rats with periodontitis. This research is an experimental study with a post-test-only control group design. Thirty rats were grouped into five groups, namely negative control (without treatment), positive control (given silk ligature 14 days), and treatments 1, 2, and 3 (given silk ligature for 14 days, followed by administration of vitamin E with doses 20, 40, and 60 IU for ten days). On the last day of the study, blood was collected to check the levels of Malondialdehyde (MDA) and gingival inflammation through the gingival index score. The results showed that the mean MDA levels in the positive control group given silk ligature alone had the highest MDA levels compared to the negative control group and all treatment groups. Administration of vitamin E significantly reduced serum MDA levels in all treatment groups (p < 0.05). Vitamin E also reduced the gingival index score compared to the positive control group in all groups. Conclusion: The administration of vitamin E can overcome oxidative stress and inflammation in periodontitis, characterized by a decrease in MDA levels and gingival score index.

 

Keywords: Vitamin E, Oxidative Stress, Inflammation, Periodontitis

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References

Arajibani, M.K., Ashemi, M.H., Ontazerifar, F.M., and Ikshit, M.D., 2010. Effect of Vitamin E and C Supplements on Antioxidant Defense System in Cardiovascular Disease Patients in Zahedan, Southeast Iran, J Nutr Sci Vitaminol 56, 436-440.

Ayala, A., Muñoz, M.F., and Argüelles, S., 2014. Lipid peroxidation: Production, metabolism, and signaling mechanisms of malondialdehyde and 4-hydroxy-2-nonenal. Oxidative Medicine and Cellular Longevity, 1-31.

Behfarnia, P., Dadmehr, M., Hosseini, S.N., and Mirghaderi, S.A., 2021. The effect of Vitamin E supplementation on treatment of chronic periodontitis, Dental Research Journal 18:62, 1-6.

Birben, E., Sahiner, U.M., Sackesen, C., Erzurum, S., and Kalayci, O., 2012. Oxidative Stress and Antioxidant Defense. WAO Journal 5, 9–19.

Carvalho, R.D.S., de Souza, C.M., Neves, J.C.D.S., Holanda-Pinto, S.A., Pinto, L.M.S., Brito, G.A.C., and de Andrade, G.M., 2013. Vitamin e does not prevent bone loss and induced anxiety in rats with ligature-induced periodontitis. Archives of Oral Biology 58, 50–58.

Genco, R.J. and Williams, R.C., 2010. Periodontal disease and overall health : a clinician’s guide. Professional Audience Communications.

Glascoe L, A., Brown S, R., Marshall L, K., and Smith R, D., 2015. Periodontal & Oral-Systemic Relationships: Reproductive Health. Austin Journal of Dentistry 2, 1–5.

Hong, J.Y., Lee, J.S., Choi, S.H., Shin, H.S., Park, J.C., Shin, S. il, and Chung, J.H., 2019. A randomized, double-blind, placebo-controlled multicenter study for evaluating the effects of fixed-dose combinations of vitamin C, vitamin E, lysozyme, and carbazochrome on gingival inflammation in chronic periodontitis patients. BMC Oral Health 19, 1-8.

Ionel, A., Lucaciu, O., Moga, M., Buhatel, D., Ilea, A., Tabaran, F., Catoi, C., Berce, C., Toader, S., and Campian, R.S., 2015. Periodontal disease induced in Wistar rats-experimental study. Human & Veterinary Medicine International-Journal of the Bioflux Society 7, 90–95.

Khoubnasabjafari, M., Ansarin, K., and Jouyban, A., 2015. Reliability of malondialdehyde as a biomarker of oxidative stress in psychological disorders. BioImpacts 5, 123–127.

Könönen, E., Gursoy, M., and Gursoy, U.K., 2019. Periodontitis: A multifaceted disease of tooth-supporting tissues. Journal of Clinical Medicine 8, 1-12.

Kurutas, E.B., 2016. The importance of antioxidants which play the role in cellular response against oxidative/nitrosative stress: Current state. Nutrition Journal 15:71, 1-22.

Liu, Z., Liu, Y., Song, Y., Zhang, X., Wang, S., and Wang, Z., 2014. Systemic oxidative stress biomarkers in chronic periodontitis: A meta-analysis. Disease Markers 2014, 1-10.

Mohd Zaffarin, A.S., Ng, S.F., Ng, M.H., Hassan, H., and Alias, E., 2020. Pharmacology and pharmacokinetics of Vitamin E: Nanoformulations to enhance bioavailability. International Journal of Nanomedicine 15, 9961-9974.

Monisha K. and Savitha, G., 2016. Assessment of Oxidative Stress in Periodontitis Patients. Journal of Pharmaceutical Sciences and Research 8, 620–622.

Nazrun, A.S., Norazlina, M., Norliza, M., and Nirwana, S.I., 2012. The anti-inflammatory role of vitamin e in prevention of osteoporosis. Advances in Pharmacological Sciences 2012, 1-7.

Quamilla, N., 2016. Stres dan Kejadian Periodontitis (Kajian Literatur). Journal of Syiah Kuala Denstistry Society 1, 161–168.

Rita, R.S., Yerizel, E., Asbiran, N., and Kadri, H., 2009. Pengaruh Ekstrak Mengkudu Terhadap Kadar Malondialdehid Darah dan Aktivitas Katalase Tikus DM yang Diinduksi Aloksan. Majalah Kedokteran Andalas 33, 54–64.

Santoso, O., 2019. Infeksi Periodontal Sebagai Faktor Risiko Kondisi Sistemik. ODONTO Dental Journal 6, 141–152.

Sharifi-Rad, M., Anil Kumar, N. v., Zucca, P., Varoni, E.M., Dini, L., Panzarini, E., Rajkovic, J., Tsouh Fokou, P.V., Azzini, E., Peluso, I., Prakash Mishra, A., Nigam, M., el Rayess, Y., Beyrouthy, M. el, Polito, L., Iriti, M., Martins, N., Martorell, M., Docea, A.O., Setzer, W.N., Calina, D., Cho, W.C., and Sharifi-Rad, J., 2020. Lifestyle, Oxidative Stress, and Antioxidants: Back and Forth in the Pathophysiology of Chronic Diseases. Frontiers in Physiology 11, 1-21.

Singh, D., Bains, V., Srivastava, R., Kumar Bains, V., Jhingran, R., and Madan, R., 2017. Oxidative Stress: A link Between Periodontal & Systemic Health Oxidative Stress: A Link between Periodontal and Systemic Health, Systemic Health Asian Journal of Oral Health & Allied Sciences 7, 14-21.

Stolzenberg-Solomon, R.Z., Sheffler-Collins, S., Weinstein, S., Garabrant, D.H., Mannisto, S., Taylor, P., Virtamo, J., and Albanes, D., 2009. Vitamin e intake, α-tocopherol status, and pancreatic cancer in a cohort of male smokers. American Journal of Clinical Nutrition 89, 584–591.

Tahan, G., Aytac, E., Aytekin, H., Gunduz, F., Dogusoy, G., Aydin, S., Tahan, V., and Uzun, H., 2011. Vitamin e has a dual effect of anti-inflammatory and antioxidant activities in acetic acid-induced ulcerative colitis in rats. Canadian Journal of Surgery 54, 333–338.

Trivedi, S., and Lal, N., 2017. Antioxidant enzymes in periodontitis. Journal of Oral Biology and Craniofacial Research 7, 54-57.

Zing, J.M., 2018. Vitamin E: Regulatory Role on Signal Transduction. Annual Review of Nutrition 35, 135-173

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