Aktivitas Antioksidan Ekstrak Etanol Daun Ganitri (Elaeocarpus Ganitrus Roxb.) dengan Metode DPPH (2,2 Difenil-1-Pikrilhidazil)

Naelaz Zukhruf Wakhidatul Kiromah(1*), Sadam Husein(2), Titi Pudji Rahayu(3)

(1) Sekolah Tinggi Ilmu Kesehatan Masyarakat Muhammadiyah Gombong
(2) Sekolah Tinggi Ilmu Kesehatan Muhammadiyah Gombong
(3) Sekolah Tinggi Ilmu Kesehatan Muhammadiyah Gombong
(*) Corresponding Author

Abstract

Free radicals are one of the cause of various diseases. The use of synthetic antioxidant compounds could prevent the effect of the free radicals, however may cause adverse effects on the human body such as impaired liver, lung, intestinal and poisoning. Therefore antioxidant from natural resources needs to be developed. The purpose of this research was to determine the antioxidant activity and IC50 value of the ethanol extract of ganitri (Elaeocarpus ganitrus Roxbs.) leaves. Ganitri leaf ethanol extract activity test was carried out using DPPH method with vitamin C as a standard. Antioxidant activity was determined as a decreas in the absorbance of DPPH at 517 nm wavelength after an addition of the extract with the concentrations of 20, 40, 80, and 100 ppm. The antioxidant acitivity measurement of the ganitri leaf extract showed that the linier regression equation obtained was y = 0.3669x + 29.546, r = 0.4573 while the IC50 value was 54,12 ppm. Based on the result, it is concluded that the ethanol extract of ganitri (Elaeocarpus ganitrus Roxb.) leaf showed was categorized as strong antioxidant.

Keywords

Antioxidant; Ganitri; DPPH; IC50

Full Text:

PDF

References

Adriani, D., & Murtisiwi, L. 2020. Uji Aktivitas Antioksidan Ekstrak Etanol 70% Bunga Telang (Clitoria ternatea L) dari Daerah Sleman dengan Metode DPPH. Pharmacon: Jurnal Farmasi Indonesia, 17(1), 70-76.

Agustina, W. and Handayani, D. 2017. Skrining Fitokimia dan Aktivitas Antioksidan Beberapa Fraksi dari Kulit Batang Jarak ( Ricinus communis L .). Jurnal Pendidikan dan Ilmu Kimia, 1(2), pp. 117–122.

Arifin, B. and Ibrahim, S. 2018. Struktur, Bioaktivitas dan Antioksidan Flavonoid. Jurnal zarah, 6(1), pp. 21–29.

Dianasari, D., & Firdiyansari, I. 2019. Potensi Ekstrak Etanol Herba Apu-Apu (Pistia stratiotes) dan Fraksi-Fraksinya Sebagai Antioksidan Dengan Metode DPPH. Pharmacon: Jurnal Farmasi Indonesia, 16(2), 83-88.

Divya, P. J., Jamuna, P. and Jyothi, L. A. 2016. Antioxidant Properties of Fresh and Processes Citrus aurantium fruit. 2.

Dubey, G. A. 2018. Effect Of Extract Of Rudraksa (Elaeocarpus ganitrus) On Parkison'S Disease and Depression. 7 (12), pp. 937–947. doi: 10.20959/wjpr201812-12697.

Floegel, A. et al. 2011. Journal of Food Composition and Analysis Comparison of ABTS / DPPH Assays to Measure Antioxidant Capacity in Popular Antioxidant Rich. Journal of Food Composition and Analysis. Elsevier Inc., 24(7), pp. 1043–1048. doi: 10.1016/j.jfca.2011.01.008.

Hasanah, M. et al. 2017. Daya Antioksidan Ekstrak dan Fraksi Daun Kopi Robusta (Coffea robusta) Terhadap Antioxidant Of Extract and Fraction Coffea robusta Leaves with Diphenylpicrylhidrazyl (DPPH) Method. 4.

Irianti, T. et al. 2015. Aktivitas Penangkapan Radikal 2-2 Difenil-1-Pikrilhidrazil (DPPH) Ekstrak Etanolik Daun Mengkudu (Morinda citrifolia L) dan Batang Brotowali (Tinospora crispa L.), Fraksi Air Serta Fraksi Air Terhidrolisis. Journal Traditional Medicine, 20(3), pp. 140–148.

Kementerian Kesehatan RI. 2014. Farmakope Indonesia. 5th edn. Jakarta: Direktorat Jendral Bina Kefarmasian dan Alat Kesehatan.

Kumar, G. and dkk. 2011. Antimicrobial Activity of Elaeocarpus ganitrus Roxb ( Elaeocarpaceae ): An in vitro Bio Technology Antimicrobial activity of Elaeocarpus ganitrus Roxb ( Elaeocarpaceae ): An In Vitro Study. Bio Technologi, 40 (May 2014), pp. 5384–5387.

Kumar, T. S. and Thayumanavan, P. 2014. Evaluation of Antioxidant Properties of Elaeocarpus ganitrus Roxb Leaves. Iranian Journal of Pharmaceutical Research. pp. 1–6.

Liochev, S. I. 2013. Reactive Oxygen Species and The Free Radical Theory of Aging. 60.

Najihudin, A., Chaerunisaa, A. and Subarnas, A. 2017. Aktivitas Antioksidan Ekstrak dan Fraksi Kulit Batang Trengguli (Cassia fistula L) dengan Metode DPPH. IJPST. 4(2).

Nugraheni. 2007. Perbandingan Aktivitas Antioksidan Ekstrak Etanol dan Ekstrak Metanol Daun Tempuyung (Sunchus arvensis L.) serta Penentuan EC50 dengan Metode DPPH (1,1-difenil-2-pikrilhidrazil). Sekolah Tinggi Ilmu Farmasi Semarang, pp. 36–39.

Panagan, A. T. 2011. Isolasi Mikroba Penghasilan Antibiotika dari Tanah Kampus Unsri Indralaya Menggunakan Media Ekstrak Tanah. Jurnal Penelitian Sains, 14(3).

Salampe, M. et al. 2019. Aktivitas Antioksidan Ekstrak Etanol Daun Beroma (Cajanus cajan ( L .) Milps ). Majalah Farmasi dan Farmakologi. 23(1), pp. 29–31.

Singh, A. K. and Kumar, S. 2018. Comparison between Compressive Strength of Rudraksha bead ( Elaeocarpus ganitrus Roxb) in Vertical and Horizontal Plane and Bead Properties. (6), pp. 38–44.

Srikanth, M. et al. 2018. Phytochemical Screening and In Vitro Anti-Oxidant Activity of Elaeocarpus. Journal Pharmacy Science 1(1), pp. 2–4.

Article Metrics

Abstract view(s): 1069 time(s)
PDF: 2896 time(s)

Refbacks

  • There are currently no refbacks.