Penghambatan Ekstrak Daun Kremah (Alternanthera sessilis) Terhadap Enzim α-amilase secara In-Vitro

Wirasti Wirasti(1*), Titi Lestari(2), Isyti'aroh Isyti'aroh(3)

(1) Prodi Sarjana Farmasi UMPP Jl.Raya Ambokembang No 8 Kedungwuni Pekalongan
(2) 
(3) 
(*) Corresponding Author

Abstract

An important treatment for type 2 diabetes is controlling blood glucose levels. α-amylase enzyme in the body plays a role in breaking down carbohydrates into starch. Control of the amylase enzyme is needed in diabetes cases Kremah plant (Alternanthera sessillis) is a plant whose leaves can be used to lower blood glucose levels because it contains secondary metabolites, one of which is flavonoids which can inhibit the α-amylase enzyme. The purpose of this study was to determine the activity and inhibition value of kremah leaves against the α-amylase enzyme. The method used in this research is the enzymatic reaction by measuring the intensity of the color using UV-Vis spectrophotometry. The IC50 value obtained by α-amylase enzyme inhibition was 101.89±7,21 μg / ml while the IC50 acarbose value was 127.17±4,42 μg / ml. These results indicate that kremah leaf extract has activity (hyperglycemia) by inhibiting complex carbohydrate hydrolyzing enzymes such as the α-inhibition enzyme α-Amylase which is good compared to acarbose because the IC50 value of the extract is smaller, so that the leaves of kremah has inhibiting of the α-amylase enzyme.

Keywords

Daun Kremah, ekstrak, α-Amylase, Penghambatan

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References

Afriyanti, T. 2015, Uji potensi antidiabetik pada ekstrak daun Garcinia mangostana L. melalui penghambatan aktifitas alfa glukosidase dan alfa amilase serta penapisan fitokimia pada ekstrak teraktif. Universitas Indonesia

Goel, R., Dask, B., Sadaf, J.G., and Deepti, K. 2012. Medicinal plants as anti-diabetics: a review. International Bulletin of Drug Research; 1 (2) :100-107

Mrinmay, D., Ashok, K.D., Mastanaiah, K., and Das, A. 2015. Evaluation of anti-diabetic activity of ethanolic extract of Alternanthera sessilis Linn. in streptozotocin-induced diabetic rats. International Journal of Pharma Sciences and Research (IJPSR); 6 (7) : 1027-1032

Pujiyanto, S., Wijanarka., Budi, R and Via, A. 2019. Aktivitas inhibitor α-amilase ekstrak etanol tanaman brotowali (Tinospora crispa L.). Bioma; 21 (2) : 91-99

Rizza, R.A. 2010. Pathogenesis of fasting and postprandial hyperglycemia in type 2 diabetes: Implications for Therapy. Diabetes Journal; 59(1): 2697-2707

Sugiwati S., Siswati S., and Efy, A. 2019. Antihyperlycemic activity of the mahkota dewa (Phaleria macrocarpa (Scheff.) Boerl.) leaf extracts as an alphaglucosidase inhibitor. Makara Kesehatan; 13 (2): 74-78

Tadera, K., Yuji, M., Kouta, T., and Tomoko, M. 2005. Inhibition of α-glukosidase and α-amylase by flavonoids. Japan : Kagoshima University

Verma, S., Madhu, .G, Harvinder, P., & Geeta, A. 2018. Diabetes mellitus treatment using herbal drugs. International Journal Of Phytomedicine; 10(1) : 1-10

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