Combination of Ketepeng Cina (Cassia alata Linn) And Lidah Buaya Buaya (Aloe vera) As Antibacterial Agent Against Staphylococcus aureus And Pseudomonas aeruginosa

Cita Hanif Muflihah(1*), Az Zahra Miftahul Husna(2), Aminah Fitriana Hamka(3)

(1) Fakultas Farmasi Universitas Muhammadiyah Surakarta
(2) Fakultas Farmasi Universitas Muhammadiyah Surakarta
(3) Fakultas Farmasi Universitas Muhammadiyah Surakarta
(*) Corresponding Author


Infection is one of the biggest health problems in Indonesia. Infection can be caused by pathogenic microorganisms such as Staphylococcus aureus and Pseudomonas aeruginosa. Treatment with antibiotics is the primary choice to manage infections caused by bacteria. Previous research showed that many plants have been tested for their antibacterial activity such as ketepeng cina (Cassia alata) and lidah buaya (Aloe vera). Cassia alata and Aloe vera alkaloids, flavonoids, anthraquinones, saponins, tannins contain similar active compounds as antimicrobials. Based on this, the purpose of this study was to determine the antibacterial activity of the combination of methanol extracts of C. alata and A. vera against S. aureus and P. aeruginosa bacteria. The antibacterial activity test was carried out using the Kirby-Bauer method. The single extract of C. alata and A. vera had the greatest antibacterial activity against S. aureus at a concentration of 10 mg/disk with zones of inhibition of 29.5±0.7 mm and 21.5±1.4 mm, respectively. The single antibacterial activity test of both extracts against P. aeruginosa bacteria showed no inhibition zone. The test results showed that the combination of 10 mg/disk C. alata methanol extract and 10 mg/disk Aloe vera could inhibit the growth of S. aureus with an average inhibition zone of 30.75±1.7 mm. However, the lower ratio of both extracts resulted in a likely antagonistic effect against S. aureus. Phytochemical screening tests showed that these two extracts had the same compounds, namely phenolics, flavonoids, anthraquinones. Contact bioautography showed that mainly phenolics, flavonoids, and anthraquinones were responsible in the antibacterial properties.


Cassia alata, Aloe vera, Staphylococcus aureus, Pseudomonas aeruginosa

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A’lana L., Sari R. and Apridamayanti P., 2017, FICI value determination of combination of Aloe vera (L) Burm.f ethanol extract with gentamycin sulphate ag, Pharm Sci Res, 4 (3), 132–142.

Bliss C.I., 1939, the Toxicity of Poisons Applied Jointly, Annals of Applied Biology, 26 (3), 585–615.

Bouarab-Chibane L., Forquet V., Lantéri P., Clément Y., Léonard-Akkari L., Oulahal N., Degraeve P. and Bordes C., 2019, Antibacterial properties of polyphenols: Characterization and QSAR (Quantitative structure-activity relationship) models, Frontiers in Microbiology, 10 (APR), 829.

Breijyeh Z., Jubeh B. and Karaman R., 2020, Resistance of Gram-Negative Bacteria to Current Antibacterial Agents and Approaches to Resolve It, Molecules, 25 (6)

CDC C. for D.C. and P., 2019, Pseudomonas aeruginosa Infection | HAI | CDC,

Dewi R. and Marniza E., 2019, Antibacterial activity of Aloe vera gel against Staphylococcus aureus, Jurnal Saintek Lahan Kering, 2 (2), 61–62.

Exner M., Bhattacharya S., Christiansen B., Gebel J., Goroncy-Bermes P., Hartemann P., Heeg P., Ilschner C., Kramer A., Larson E., Merkens W., Mielke M., Oltmanns P., Ross B., Rotter M., Schmithausen R.M., et al., 2017, Antibiotic resistance: What is so special about multidrug-resistant Gram-negative bacteria?, GMS Hygiene and Infection Control, 12,

Fatmawati S., Yuliana, Purnomo A.S. and Abu Bakar M.F., 2020, Chemical constituents, usage and pharmacological activity of Cassia alata, Heliyon, 6 (7)

Gupta V. and Datta P., 2019, Next-generation strategy for treating drug resistant bacteria: Antibiotic hybrids, The Indian Journal of Medical Research, 149 (2), 97.

Hodges N., 2013, Antibiotics and synthetic antimicrobial agents: their properties and uses, Dalam Denyer, S. P. et al., eds. Hugo and Russell’s Pharmaceutical Microbiology, iley-Blackwell A John Wiley & Sons, Ltd., Publication, West Sussex, United Kingdom, p. 185.

Hudzicki J., 2012, Kirby-Bauer Disk Diffusion Susceptibility Test Protocol Author Information, American Society For Microbiology, (December 2009), 1–13. :

Jain S., Rathod N., Nagi R., Sur J., Laheji A., Gupta N., Agrawal P. and Prasad S., 2016, Antibacterial Effect of Aloe Vera Gel against Oral Pathogens: An In-vitro Study, Journal of Clinical and Diagnostic Research : JCDR, 10 (11), ZC41.

Jangnga I.D., Kambaya P.P. and Kosala K., 2018, In vitro antibacterial activity and bioautography-thin layer chromatography analysis of Srikaya (Annona Squamosa L) leaves ethanolic extracts against Enterococcus faecalis, ODONTO : Dental Journal, 5 (2), 102.

Khan M.R., Kihara M. and Omoloso A.D., 2001, Compositional chemistry of Cassia alata seeds, Fitoterapia, 72 (3), 561–564.

Marliana S.D., Suryanti V. and Suyono, 2005, The phytochemical screening and thin layer chromatograpy analysis of ethanolic extracts of chayote ( Sechium edule Jacq . Swartz .), Biofarmasi, 3 (1), 26–31.

Miller S.I., 2016, Antibiotic Resistance and Regulation of the Gram-Negative Bacterial Outer Membrane Barrier by Host Innate Immune Molecules, mBio, 7 (5)

Muflihah C.H., Haryoto H. and Indrayudha P., 2020, Cytotoxic Assay of Semipolar Fraction Of Ethanolic Extract From Sugar Apple (Annona Squamosa L.) Stem Bark on T47D Cells, Pharmacon: Jurnal Farmasi Indonesia, 17 (2), 148–156.

Nurhayati S., Ratnasari D. and Handayani R.P., 2019, Formulation and physical stability assay of Ketepeng Cina gel (Cassia alata L.) with Aloe-based gel (Aloe vera L.), Journal of Holistic and Health Sciences, 3 (2)

Rahardjo M., Koendhori E.B. and Setiawati Y., 2017, Antibacterial test of Aloe vera ethanol extracts against Staphylococcus aureus, Jurnal Kedokteran Syiah Kuala, 17 (2), 65–70.

Ramadhani N., Ramadhani N., Samudra A.G. and Armando J., 2017, Identification of the ethanolic extract of Mimba leaves ( Azadirachta indica A . Juss ) chemical content as antibacterial using TLC-bioautography against Staphylococcus aureus and Escherichia coli, Jurnal Ilmiah Ibnu Sina, 2 (1), 74–81.

Sánchez M., González-Burgos E., Iglesias I. and Gómez-Serranillos M.P., 2020, Pharmacological Update Properties of Aloe Vera and its Major Active Constituents, Molecules, 25 (6) : /pmc/articles/PMC7144722/.

Sesa O.E., Sulastry T. and Muharram, 2014, Secondary metabolites isolation and identification of Ketepeng Cina ( Cassia alata Linn ) leaves methanolic extracts, Jurnal Chemica, 15 (1), 136–143.

Suganya T., Packiavathy I.A.S.V., Aseervatham G.S.B., Carmona A., Rashmi V., Mariappan S., Devi N.R. and Ananth D.A., 2022, Tackling Multiple-Drug-Resistant Bacteria With Conventional and Complex Phytochemicals, Frontiers in Cellular and Infection Microbiology, 12, 671.

Tauran Id P.M., Djaharuddin Id I., Id U.B., Nurulita A., Katu S., Muchtar F., Meutia Pelupessy N., Hamersid R.L., Day N.P.J., Arif M. and Limmathurotsakulid D., 2022, Excess mortality attribuTable to antimicrobial-resistant bacterial bloodstream infection at a tertiary-care hospital in Indonesia, PLOS Global Public Health, 2 (7)

Toh S.C., Lihan S., Bunya S.R. and Leong S.S., 2023, In vitro antimicrobial efficacy of Cassia alata (Linn.) leaves, stem, and root extracts against cellulitis causative agent Staphylococcus aureus, BMC Complementary Medicine and Therapies, 23 (1), 85.

Tong S.Y.C., Davis J.S., Eichenberger E., Holland T.L. and Fowler V.G., 2015, Staphylococcus aureus Infections: Epidemiology, Pathophysiology, Clinical Manifestations, and Management, Clinical Microbiology Reviews, 28 (3), 603.

Wagner H. and Bladt S., 1996, Plant Drug Analysis: A Thin Layer Chromatography Atlas. 2nd Edition, Springer Berlin Heidelberg, Berlin.

Xie Y., Yang W., Tang F., Chen X. and Ren L., 2015, Antibacterial Activities of Flavonoids: Structure-Activity Relationship and Mechanism, Current Medicinal Chemistry, 22, 132–149.

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