Reducing Activity of Interleukin-8 (IL-8) Extract of Sea Cucumber (Holothuroidea sp.) in Experimental Animals Induced by Carrageenan

Mursalina Nanda Aurum(1), Andi Suhendi(2*), Muhammad Da'i(3), Ahmad Fauzi(4)

(1) Faculty of Pharmacy, Universitas Muhammadiyah Surakarta
(2) Faculty of Pharmacy, Universitas Muhammadiyah Surakarta
(3) Faculty of Pharmacy, Universitas Muhammadiyah Surakarta
(4) Faculty of Pharmacy, Universitas Muhammadiyah Surakarta
(*) Corresponding Author

Abstract

Interleukin-8 is a pro-inflammatory cytokine that plays a role in the inflammatory response. Sea cucumbers (Holothuria sp.) are used as alternative medicines that have anti-inflammatory activity. The study aims to determination of Sea cucumber (Holothuria sp.) as anti inflammation activty based on capability of reducing interleukin-8 (IL-8) on Rats induced by Carrageenan. Experimental research used was a post-test-only control group design. A total of 25 rats were divided into 5 groups, namely negative control, positive control (Na. Diclofenac), and treatment groups given Sea cucumber extract (doses of 45, 90, and 180 mg/ g BW). Carrageenan-induced mouse paw edema model was used for induction of inflammation. IL-8 of Paw tissue were measured by an ELISA reader at 450 nm wavelength. The result showed that treatrment groups has anti-inflammation activity based on IL-8 levels compared to negative and positive controls. The lowest levels of IL-8 showed at a dose of 90 mg/g BW. Based on One-way ANOVA, there were no significant differences of between groups. Sea cucumber has anti-inflammation activity by reducing IL-8 at all dose tested

Keywords

interleukin-8 (IL-8); anti-inflammatory; sea cucumber extract; carrageenan; ELISA

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