Correlation of Phenolic Content of Multiflora Honey from Malang and Alastuwo to Activity Antioxidant Using DPPH (2.2-Diphenyl-1-Pickrylhydrazyl)
Ahwan Abdul(1*)(1) Sahid Surakarta University
(*) Corresponding Author
Abstract
Honey contains various compounds that function as antioxidants, one of which is phenolic compounds. The purpose of this study was to determine the correlation between the phenolic content of multiflora honey from Malang and Alastuwo on antioxidant activity using the DPPH method (2,2-Diphenyl-1-picrylhydrazyl). Qualitative test of phenolic compounds with FeCl3 color reagent, determination of total phenolic content by UV-Vis spectrophotometry using Folin-Ciocalteu method and antioxidant activity test using DPPH method (2,2-Diphenyl-1-picrylhydrazyl). The results showed that qualitatively Malang and Alastuwo honey contained phenolic compounds. The total phenolic content of Alastuwo honey was 0.0278 ± 0.0010 mg GAE/g and Malang honey was 0.0301 ± 0.0004 mg GAE/g. The results of the antioxidant activity test showed that Alastuwo honey had weak antioxidant activity (IC50 of 393.37 ± 10.28 ppm), Malang honey had moderate antioxidant activity (IC50 of 217.20 ± 6.61 ppm) and vitamin C had strong antioxidant activity. (IC50 is 2.22 ± 0.19 ppm). The total phenolic content of Alastuwo and Malang honey correlates with its antioxidant activity.
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Abdul, A., 2020. Uji Aktivitas Antioksidan Ekstrak Etanol Daun Adas (Foeniculum Vulgare Mill) Dengan Metode DPPH Dan FRAP. Pharmed J. Pharm. Sci. Med. Res. 3, 43–54.
Akuru, U.B., Amadi, B.A., 2018. Phytochemicals and antioxidant properties of some selected medicinal plants. J. Pharmacogn. Phytochem. 7, 283–285.
Arawwawala, M., Hewageegana, S., 2017. Health benefits and traditional uses of honey: A review. J. Apitherapy 2.
Ardiansyah, 2011. Antioksidan dan Peranannya bagi Kesehatan. Artik. Iptek.
Babarinde, G.O., Babarinde, S.A., Adegbola, D.O., Ajayeoba, S.I., 2011. Effects of harvesting methods on physicochemical and microbial qualities of honey. J. Food Sci. Technol. 48, 628–634.
Bintoro, V.P., Legowo, A.M., Purnomoadi, A., Setiani, B.E., 2015. Garlic antioxidant (Allium sativum L.) to prevent meat rancidity. Procedia Food Sci. 3, 137–141.
Bogdanov, S., Haldimann, M., Luginbühl, W., Gallmann, P., 2007. Minerals in honey: environmental, geographical and botanical aspects. J. Apic. Res. 46, 269–275.
Buba, F., Gidado, A., Shugaba, A., 2013. Analysis of biochemical composition of honey samples from North-East Nigeria. Biochem Anal Biochem 2, 139.
Chua, L.S., Rahaman, N.L.A., Adnan, N.A., Eddie Tan, T.T., 2013. Antioxidant activity of three honey samples in relation with their biochemical components. J. Anal. Methods Chem. 2013.
Eleazu, C.O., Iroaganachi, M.A., Eleazu, K.C., Okoronkwo, J.O., 2013. Determination of the physico-chemical composition, microbial quality and free radical scavenging activities of some commercially sold honey samples in Aba, Nigeria:’the effect of varying colours. Int. J. Biomed. Res. 4, 32–41.
Hussein, S.Z., Yusoff, K.M., Makpol, S., Yusof, Y.A.M., 2011. Antioxidant capacities and total phenolic contents increase with gamma irradiation in two types of Malaysian honey. Molecules 16, 6378–6395.
Khalil, M.I., Sulaiman, S.A., Boukraa, L., 2010. Antioxidant properties of honey and its role in preventing health disorders. Open Nutraceuticals J. 3.
Kumazawa, S., Okuyama, Y., Murase, M., Ahn, M.-R., Nakamura, J., Tatefuji, T., 2012. Antioxidant activity in honeys of various floral origins: Isolation and identification of antioxidants in peppermint honey. Food Sci. Technol. Res. 18, 679–685.
Lachman, J., Hejtmankova, A., Sýkora, J., Karban, J., Orsak, M., Rygerova, B., 2010. Contents of major phenolic and flavonoid antioxidants in selected Czech honey. Czech J. Food Sci. 28, 412–426.
Mahantesh, S.P., Gangawane, A.K., Patil, C.S., 2012. Free radicals, antioxidants, diseases and phytomedicines in human health: Future perspects. World Res. J. Med. Aromat. Plants 1, 6–10.
Muawanah, A., Wardhani, P., 2014. Aktivitas Antikanker dan Antioksidan Madu di Pasaran Lokal Indonesia. J. Ilmu Pertan. Indones. 19, 136–144.
Nafisah, M., Tukiran, S., Hidayati, N., 2014. Uji Skrining Fitokimia Pada Ekstrak Heksan, Kloroform dan Metanol dari Tanaman Patikan Kebo (Euphorbiae hirtae), in: Prosiding seminar nasional kimia. hal. 281–282.
Nagai, T., Tanoue, Y., Kai, N., Suzuki, N., 2012. Functional property of honey from Echium vulgare. Food Nutr. Sci. 3, 614–620.
Nayik, G.A., Nanda, V., 2015. Physico-chemical, enzymatic, mineral and colour characterization of three different varieties of honeys from Kashmir valley of India with a multivariate approach. Polish J. Food Nutr. Sci. 65.
Pontis, J.A., Costa, L.A.M.A. da, Silva, S.J.R. da, Flach, A., 2014. Color, phenolic and flavonoid content, and antioxidant activity of honey from Roraima, Brazil. Food Sci. Technol. 34, 69–73.
Qonitah, F., Ahwan, A., 2019. AKTIVITAS ANTIOKSIDAN DAN KANDUNGAN FENOLIK TOTAL FRAKSI N-HEKSAN DAN KLOROFORM DAUN JERUK PURUT (Citrus hystrix). J. Ilm. As-Syifaa 11, 99–102.
Ratnayani, K., Laksmiwati, M., Septian, N.P.I., 2012. Kadar total senyawa fenolat pada madu randu dan madu kelengkeng serta uji aktivitas antiradikal bebas dengan metode DPPH (Difenilpikril Hidrazil). J. Kim. 6, 163–168.
Rosana, M., Ahwan, A., Qonitah, F., 2021. Uji Kualitatif Aktivitas Antioksidan Ekstrak Etanol Propolis. Lumbung Farm. J. Ilmu Kefarmasian 2, 154–157.
Saputri, D.S., Putri, Y.E., 2017. Aktivitas Antioksidan Madu Hutan di Beberapa Kecamatan di Kabupaten Sumbawa Besar. J. Tambora 2.
Syaifuddin, S., 2015. Uji aktivitas antioksidan bayam merah (alternanthera amoena voss.) segar dan rebus dengan metode DPPH. UIN Walisongo.
Werdhasari, A., 2014. Peran antioksidan bagi kesehatan. J. Biotek Medisiana Indones. 3, 59–68.
Yaoa, L., Jiang, Y., Singanusong, R., Datta, N., Raymont, K., 2005. Phenolic acids in Australian Melaleuca, Guioa, Lophostemon, Banksia and Helianthus honeys and their potential for floral authentication. Food Res. Int. 38, 651–658.
Yuslianti, E.R., 2018. Pengantar Radikal Bebas dan Antioksidan. Deepublish.
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