Antioxidant Activity of Sunflower (Helianthus Annuus l.) Ethanolic Extract with DPPH Method and Determination of Total Phenolic and Flavonoid Levels
Salwa Salwa(1*)(1) Pharmacy Faculty, Universitas Muhammadiyah Surakarta
(*) Corresponding Author
Abstract
Sunflower (Helianthus annuus L.) is believed to have antioxidant properties. Currently, there are many studies examining compounds that have benefits as antioxidants and are safe for health. However, the sunflower only examined the antioxidants in sunflower seeds. This study aims to determine the antioxidant activity of ethanol extract of flowers, bark, and leaves of sunflowers. The antioxidant activity test using the DPPH method showed the highest antioxidant activity was found in the leaf extract (Helianthus annuus L.) with an IC50 value of 48.841 ppm. The highest phenolic content was found in leaf samples with a value of 35.149 mg gallic acid equivalent/gram extract and the highest determination of flavonoid levels was found in leaf samples with a value of 10.917 mg quercetin equivalent/gram extract.
Keywords
Full Text:
PDFReferences
Ahmad, AR, & Asrifa, WO (2014). Study of Antioxidant activity and determination of Phenol and Flavonoid content of Pepino's Leaf extract (Solanum muricatum Aiton). In the International Journal of PharmTech Research.
Alfian, R., & Susanti, H. (2012). Determination of Total Phenolic Content of Methanol Extract of Red Rosella Flower Petals (Hibiscus Sabdariffa Linn) With Spectrophotometric Variations in Growing Places. Pharmaciana, 2(1).
Ali, GM (2014). Evaluation of Antioxidant Activity of Ethanolic Extract from Irradiated Sunflower (Helianthus Annuus L.) Seeds Hull. Journal of Natural Sciences Research, Vol 4(1), 2225–2921.
Aminah, A., & Abidin, Z. (2017). Determination of Total Flavonoid Content of Avocado Peel (Persen Americana Mill.) Ethanol Extract with UV-Vis Spectrophotometric Method. Journal of Indonesian Fitofarmaka, 4(2), 226–230.
Chang, C., Yang, M.H., Wen, H.M., and Chern, J. (2002). Estimation of Total Flavonoid Content in Propolis by Two Complementary Colorimetric Methods. Journal of Food and Drug Analysis, Vol 10(No 3), 178–182.
Das, N., Islam, MD., Jahan, N., Islam, MS., Khan, A., Islam, MR., and Parvin, MS (2014). Antioxidant activities of ethanol extracts and fractions of Crescentia cujete leaves and stem bark and the involvement of phenolic compounds. BMC Complementary and Alternative Medicine, Vol 14.
Dewatisari, WF, Rumiyanti, L., & Rakhmawati, I. (2018). Yield and Phytochemical Screening on Leaf Extract of Sanseviera sp. Yield and Phytochemical Screening using Leaf extract of Sansevieria Sp . Journal of Applied Agricultural Research, 17(3), 197–202.
Djapiala, Fera Yusniarti., et al and Menntang, F. (2013). Total phenol content in seaweed (Caulerpa racemosa) which has potential as an antioxidant. Fishery Product Technology Media.
Dwivedi, A., and Sharma GN, KA (2015). Research Articles. School of Pharmaceutical Sciences, Jaipur National University, Jaipur, Rajasthan, India Received., 6(1), 118–124.
Dwivedi A., SG (2014). A Review on Heliotropism Plant : Helianthus annuus L . 3(2), 149–155.
Endrini, Susi., et al. (2009). Antioxidant activity and cytotoxic effect of Kola extract (Cola nitida) on liver cancer cell culture (HepG-2) Antioxidant activities and cytotoxic effect of Cola nut. Yarsi Journal of Medicine, 17(1), 40–44.
Hanani, E., Munim, A., & Sekarini, R. (2005). Identification of Antioxidant Compounds in Callyspongia Sp Sponges from the Thousand Islands. Pharmaceutical Science Magazine, 2(3), 127–133.
Ibrahim, MH, & Jaafar, HZE (2012). Primary, secondary metabolites, H 2O 2, malondialdehyde and photosynthetic responses of Orthosiphon stimaneus benth. to different irradiance levels. Molecules, 17(2), 1159–1176.
Irwanta, D. (2014). Determination of total phenolic content and antioxidant activity test using dpph method, thesis. Scientific Journal, 4(1), 23–28.
Juniarti, R., & Herdiana, Y. (2003). Review Article : Extract Activity of Helianthus Annuus L. Farmaka, Vol. 15 Nos, 195–199.
Kurniati, Dian., and indarningsih, F. (2007). Study of the Effect of Heating on Antioxidant Activity of Noni (Morinda Citrifolia) as an Alternative Functional Food Source. 3(1), 20–25.
Lim, YY, & Murtijaya, J. (2007). Antioxidant properties of Phyllanthus amarus extracts as affected by different drying methods. LWT - Food Science and Technology, 40(9), 1664–1669.
Lindawati, NY, & Ma'ruf, SH (2020). Determination of Total Flavonoid Content of Red Bean (Phaseolus Vulgaris L.) Ethanol Extract with Visible Spectrophotometric Colorimetric Complex Method. Manuntung Scientific Journal, 6(1), 83.
Lukman, Hilmia., et al and Retnaningtyas, Y. (2016). Determination of Flavonoid Levels in Plant Leaf Extracts Using NIR and Chemometric Methods. Health Library E-Journal, 4, 8–13.
Lung, JKS and Destiani, D. (2018). Antioxidant Activity Test of Vitamins A, C, E with DPPH Method. Farmaka, 15(1), 53–62.
Molyneux, P. (2004). The use of the stable free radical diphenylpicryl-hydrazyl ( DPPH ) for estimating antioxidant activity. Songklanakarin Journal of Science and Technology, vol 50.
Sahala, Aldo and C, J, S. (2012). Antioxidant activity test and determination of total phenolic content of water fraction of ketapang leaves (Pharmacy and Community Science, 9(2), 91–97.
Sayuti, K., and Yenrina, R. (2015). Natural and Synthetic Antioxidants. Andalas University Press. Matter. 10-14.
Tiwari, P., et al and Kaur, H. (2017). Phytochemical Screening and Extraction : A Review. Hepatology, 66(6), 1866–1884.
Wahdaningsih, S., et al. (2017). Determination of Total Phenolic and Total Flavonoid Levels of Methanol Extract and Ethyl Acetate Fraction of Red Dragon Fruit Skin (Hylocereus Polyrhizus (FaCWeber) Britton and Rose). Pharmacon, 6(3), 295–301.
Article Metrics
Abstract view(s): 871 time(s)PDF: 748 time(s)
Refbacks
- There are currently no refbacks.