Inhibition Activity of Leaves, Flower and Root Extracts of Ruellia tuberosa L on α-Glucosidase Enzymes
Andi Suhendi(1*), Agus Salim Maulana(2)(1) (SCOPUS ID: 56678616200) Pharmaceutical Analysis, Faculty of Pharmacy, Universitas Muhammadiyah Surakarta
(2) Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Muhammadiyah Surakarta
(*) Corresponding Author
Abstract
Previous study stated that the herba of Ruellia tuberosa L has inhibition activity on α-glucosidase enzymes. The leaves, flowers, and roots of Ruellia tuberosa L contain flavonoids but have different activities. This study aims to determine the inhibitory activity by the leaves, flowers and roots of Ruellia tuberosa on the α-glucosidase enzyme. Extraction was performed by maceration method using ethanol 96% as solvent. Inhibition activity was measured based on the formation of a yellow solution of p-nitrophenol compound as a result of the reaction between p-nitrophenyl-α-D-glucopyranoside (as substrate) and α-glucosidase enzyme at a wavelength of 405 nm. The system reaction of inhibition study is divided into two reactions, namely S0 (contains substrates without enzyme) and S1 (contain substrate and enzyme). The results showed that inhibition activities of leaves, flower and root extract were 9.97 ± 4.97 % at 7.8125 µg/mL concentration of extract; 13.98 ± 5.18 % at 7.8125 µg/mL concentration of extract; 99.08 ± 0.12 % at 31.25 µg/mL concentration of extract, respectively. Based on data, the extract of leaves, flowers and roots of Ruellia tuberosa L. has the potential as an inhibitor of the enzyme α-glucosidase.
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Amri A.D.F., 2014, Uji aktivitas antidiabetes dari ekstrak etanol 70% tumbuhan pecah beling hutan(Ruellia tuberosa L.) menggunakan metode penghambatan enzim α-glukosidase secara In Vitro, Skripsi. Fakultas Kedokteran dan Ilmu Kesehatan. Program Studi Farmasi. UIN Syarief Hidayatullah, Jakarta.
Ankita Bachhawat J., Shihabudeen M.S. and Thirumurugan K., 2011, Screening of Fifteen Indian ayurvedic plants for alpha-glucosidase inhibitory activity and enzyme kinetics, International Journal of Pharmacy and Pharmaceutical Sciences, 3 (SUPPL. 4), 267–274.
Chothani D.L. and Mishra S.H., 2012, In vitro antioxidant activity of Ruellia tuberosa root extracts, Free Radicals and Antioxidants, 2 (4), 38–44.
Chothani D.L., Patel M.B., Mishira S.H. and Vaghasiya H.U., 2010, Review on Ruellia tuberosa (cracker plant), Pharmacognosy Journal, 2 (12), 506–512.
DEPKES RI, 2000, Parameter standar umum ekstrak tumbuhan obat, 1-68
Feng J., Yang X.W. and Wang R.F., 2011, Bio-assay guided isolation and identification of alpha-glucosidase inhibitors from the leaves of Aquilaria sinensis, Phytochemistry, 72 (2–3), 242–247.
Hanani E., Mun A. and Sekarini R., 2005, Identifikasi senyawa antioksidan dari kepulauan seribu, Majalah Ilmu Kefarmasian, II (3), 127–133.
Kemenkes RI, 2014, Situasi dan Analisis Diabetes, Pusat Data dan Informasi Kementerian Kesehatan RI, 2.
Laily Kurniawati A.N., Aulanni’am, Srihardyastutie A. and Safitri A., 2018, The Effects of Root Extract Ruellia tuberosa L on Histopathology and Malondialdehyde Levels on the Liver of Diabetic Rats, IOP Conference Series: Materials Science and Engineering, 299, 12022.
Li Y., Wen S., Kota B.P., Peng G., Li G.Q., Yamahara J. and Roufogalis B.D., 2005, Punica granatum flower extract, a potent α-glucosidase inhibitor, improves postprandial hyperglycemia in Zucker diabetic fatty rats, Journal of Ethnopharmacology, 99 (2), 239–244.
Loranza B., 2012, Uji Penghambatan Aktivitas Enzim Alfa-glukosidase dan Identifikasi Golongan Senyawa Kimia dari Fraksi Teraktif Daun Buni (Antidesma bunius L.), Skripsi. Fakultas Matematika dan Ilmu Pengetahuan. Program Studi Farmasi. Universitas Indonesia, Depok.
Mun’im A., Katrin, Azizahwati, Andriani A., Mahmudah K.F. and Mashita M., 2013, Screening of α- Glucosidase Inhibitory Activity of Some Indonesian Medicinal Plants, International J. Med. Arom. Plants, 3 (2), 144–150.
Najib A., Hartati S. and Elya B., 2011, In vitro bioassay of n-buthanol isolate of Acorus calamus L. on inhibitory of activity a-glucosidase, International Journal of PharmTech Research, 3 (4), 2085–2088.
Nampoothiri S. V., Prathapan A., Cherian O.L., Raghu K.G., Venugopalan V. V. and Sundaresan A., 2011, In vitro antioxidant and inhibitory potential of Terminalia bellerica and Emblica officinalis fruits against LDL oxidation and key enzymes linked to type 2 diabetes, Food and Chemical Toxicology, 49 (1), 125–131.
Rajan M., Kumar V.K., Kumar P.S., Swathi K.R. and Haritha S., 2012, Research Article Antidiabetic, antihyperlipidaemic and hepatoprotective activity of methanolic extract of Ruellia tuberosa Linn leaves in normal and alloxan induced diabetic rats, Journal of Chemical and Pharmaceutical Research, 4 (6), 2860–2868.
Saifudin A., 2014, Senyawa Alam Metabolit Sekunder Teori, Konsep, dan Teknik Pemurnian, 1st ed., deepublish, Yogyakarta.
Shahwara D., Ullah S., Ahmad M., Ullah S., Ahmad N. and Khan M.A., 2011, Hypoglycemic activity of Ruellia tuberosa linn (Acanthaceae) in normal and alloxan-induced diabetic rabbits, Iranian Journal of Pharmaceutical Sciences, 7 (2), 107–115.
Sugiwati S., Setiasih S. and Afifah E., 2009, Antihyperglycemic Activity of The Mahkota dewa [Phaleria macrocarpa (Scheff.) Boerl.] Leaf Extract As An Alpha-Glucosidase Inhibitor, J Logika, 13 (2), 74–78.
Tadera K., Inami Y.M., Akamatsu K.T. and Atsuoka T.M., 2006, Inhibition of Alpha-Glucosidase and Alpha-Amylase by Flavonoids, J Nutr Sci Vitaminol, 52, 149–153.
Vankar P.S. and Srivastava J., 2010, Evaluation of Anthocyanin Content in Red and Blue Flowers, International Journal of Food Engineering, 6(4), 1-13.
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