Cytotoxicity of Methanol Leaf Extract of Artocarpus Altilis, Artocarpus Heterophyllus, and Artocarpus Camansi Against MCF-7 Breast Cancer Cells

H. Haryoto(1*), Pratiwi Widowati(2)

(1) Faculty of Pharmacy, Universitas Muhammadiyah Surakarta
(2) 
(*) Corresponding Author

Abstract

Artocarpus had been used as traditional medicine for cancer, cirrhosis, malaria, hypertension, and diabetes. This research aimed to evaluate the cytotoxic activity of methanol leaf extract of Artocarpus altilis, Artocarpus heterophyllus, and Artocarpus camansi against MCF-7 breast cancer cell. The leaf extracts of Artocarpus altilis, Artocarpus heterophyllus, and Artocarpus camansi were obtained by the method of maceration with methanol. Phytochemical screening was
performed by Thin Layer Chromatography with the use of silica GF-254 for stationary phase and ethyl acetate: n-hexane (2:8) for mobile phase. The cytotoxic test was conducted by MTT with diverse concentrations of extract, namely 500, 250, 125, 62.5, and 31.25 μg/mL. The results of cytotoxic test were measured by ELISA reader. Based on phytochemical screening, tannin, and alkaloids were found in three extracts. Cytotoxic test demonstrated that those extracts had low cytotoxic activity against MCF-7 breast cancer cells, with IC50 of 120.85; 339.46 and 530.88 μg/mL, respectively, for Artocarpus altilis, Artocarpus heterophyllus, and Artocarpus camansi.

Full Text:

PDF

References

Apsari K., 2007, Isolasi dan Karakterisasi Senyawa Kimia dari Ekstrak n-Heksan Kulit Batang

Kluwih (Artocarpusaltilis Park) serta Efek Sitotoksiknya terhadap Sel HeLa, Phytochemistry,

(2), 45–51.

Arung E.T., Yoshikawa K., Shimizu K. and Kondo R., 2010, Isoprenoid-substituted Flavonoids

from Wood of Artocarpus heterophylluson B16 Melanoma Cells: Cytotoxicity and Structural

Criteria, Fitoterapia, 81 (2), 120–123.

CCRC, 2013, Uji Sitotoksik Metode MTT, Accessed on: http://ccrc.farmasi.ugm.ac.id/ [at 15

May 2016].

Haidara K., Zamir L., Shi Q. and Batist G., 2005, The Flavonoid Casticin Has Multiple

Mechanisms of Tumor Cytotoxicity Action, Cancer Letters, 1–11.

Jamalzadeh L., Ghafoori H., Sariri R., Rabuti H., Nasirzade J., Hasani H and Aghamaali M.R.,

, Cytotoxic Effects of Some Common Organic Solvents on MCF-7, RAW-264.7 and

Human Umbilical Vein Endothelial Cells, Avicenna Journal of Medical Biochemistry, In press.

Kemenkes, 2014, Hilangkan Mitos tentang Kanker, Accessed on: http://www.depkes.go.id/

article/print/201407070001/hilangkan-mitos-tentang-kanker.html [at 15 May 2016].

Kemenkes, 2015, Stop Kanker: Situasi Penyakit Kanker, infodatin-Kanker, p.3.

Khademvatan S., Eskandari A., Saki J., Foroutan-Rad M., Khademvatan S., Eskandari A., Saki

J. and Foroutan-Rad M., 2016, Cytotoxic Activity of Holothuria leucospilota Extract against

Leishmania infantum In Vitro, Advancesin Pharmacological Sciences, 2016, 1-6.

Khan M.R., Omoloso A.D and Kihara M., 2003, Antibacterial Activity of Artocarpus heterophyllus,

Fitoterapia, 74 (5), 501–505.

Moura BurciL., Bezerrada Silva C., de Oliveira M., vity of Artocarpus heterophyllus, Fitoterapia,

(5), 501–505.

Markham, K.R., 1988, Cara Mengidentifikasi Flavonoid, (Trans. Kosasih Padmawinata),

Bandung: Penerbit ITB.Mohan K., Jeyachandran R., and Mohan Assistant Professor K.,

, Alkaloids as Anticancer Agents, Annals of Phytomedicine, 1 (1), 46–53.

Dalarmi L., Maria Warumby Zanin S., Gomes Miguel O., de Fátima Gaspari Dias J. and

Dallarmi Miguel M., 2015, Determination of Antioxidant, Radical Scavenging Activity and

Total Phenolic Compounds of Artocarpus heterophyllus (Jackfruit) Seeds Extracts, Journal of

Medicinal Plants Research,8(40),1013–1020.

Pradhan C., Mohanty M. and Rout A., 2012, Phytochemical Screening and Comparative

Bioefficacy Assessment of Artocarpus altilis Leaf Extracts for Antimicrobial Activity,

Frontiers in Life Science, 6 (3–4), 71–76.

Saifudin A., 2014, Senyawa Alam Metabolit Sekunder (Teori, Konsep, dan Teknik Pemurnian),

Yogyakarta: Deepublish.Saravanan R., Brindha P. and Ramalingam S., 2014, A Review on

the Role of Phytoconstituents in Breast Cancer Cells, International Journal of Pharm Tech

Research, 6 (2), 799–808.

Shiezadeh F., Mousavi S.H., Amiri M.S., Iranshahi M., Tayarani-Najaran Z. and Karimi G., 2013,

Cytotoxic and Apoptotic Potential of Rheum turkestanicum Janisch Root Extract on Human

Cancer and Normal Cells., Iranian journal of pharmaceutical research :IJPR, 12 (4), 811–9.

Stærk D., Lykkeberg A.K., Christensen J., Budnik B.A. and AbeF., 2002, InVitro Cytotoxic Activity

of Phenanthroindolizidine Alkaloids from Cynanchum vincetoxicum and Tylophoratanakae

against Drug-Sensitive and Multidrug-Resistant Cancer Cells, Journal of Natural Products,

(3), 1299–1302.

Tantengco O.A.G. and Jacinto S.D., 2015, Cytotoxic Activity of Crude Extracts and Fractions

from Premnaodorata (Blanco), Artocarpus camansi (Blanco) and Gliricidiasepium (Jacq.)

Against Selected Human Cancer Cell Lines, Asian Pacific Journal of Tropical Biomedicine, 5

(12), 1037–1041.

Tehubijuluw H. and Tenggara A., 2013, Screening of Phytochemicals and Bioactivity Test of

The Leaves Breadfruit (Artocarpus altilis), Ind. J. Chem. Res., 1, 28–32.

Wang Y.,Xu K.,Lin L.,PanY.and Zheng X.,2007, Geranyl Flavonoids from The Leaves of

Artocarpus altilis, Phytochemistry, 68 (9),1300–1306.

DOI: 10.23917/jnhm.v1i1.5737

Article Metrics

Abstract view(s): 733 time(s)
PDF: 796 time(s)

Refbacks

  • There are currently no refbacks.