Ketahanan Eksplan Embrio Ayam Dalam Media In Vitro

Atik Kurniawati(1*), Dwi Listyorini(2)

(1) Poltekkes Kemenkes Malang
(2) Universitas Negeri Malang
(*) Corresponding Author

Abstract

Science has implemented the cell cultures as the primary for research such as a model system, toxicity tests, cancer, viruses, cell-based industries, genetic counseling, genetic engineering, gene therapy, drug screening and the latest is invitro meat. The purpose of this study was to determine the resistance of chicken embryo eksplan cells in invitro media. The research design was in the form of laboratory experiments. Explants taken 4 day old chick embryos, which were cultured in MEM media + serum. The results showed fibroblast cell cultures which survive 2 days. The most influential factor is the environment that is less supportive of the growth of explant chicken embryo cell

References

Ferguson MWJ, Leigh IM. (1998). Wound healing. Dalam: Champion RH, Burton JL, Burns DA, Breathnach SM, editor. Textbook of Dermatology. Edisi ke-6. London: Blackwell Science Ltd,; 337-43

Freshney RI. (2005). Culture of animal cells: a manual of basic tehnique. 5th ed.Willeyand Son,Inc.

Kadim, I. T., Mahgoub, O., Baqir, S., Faye, B., & Purchas, R. (2015). Cultured meat from muscle stem cells: A review of challenges and prospects. Journal of Integ-rative Agri culture, 4(2), 222-233.

Listyorini, Dwi. (2011). Kultur Jaringan Hewan: Metode Pengamatan dan Perlakuan Sel, Jaringan dan Organ Hewan secara In Vitro (Edisi Revisi). Jakarta: FMIPA UM

Lodish, H., Berk, A., Zipursky, S. L., Matsudaira, P., Baltimore, D., & Darnell, J. (2000). Molecular cell biology 4th edition. National Center for Biotechnology Information, Bookshelf, 9.

Ma'at, S. (2019). Teknik dasar kultur sel. Airlangga University Press.

Ryan, J. A. (2008). Introduction to Animal Cell Culture. Corning Incorporated. Life Sciences, Chelmsford St, 3-8.

Winterman, D. (2012). Future foods: what will we be eating in 20 years’ time. BBC News Magazine, 30th July,(accessed August 14, 2013),[available at www. bbc. co. uk/news/magazine].

Syahidah, H. N., & Hadisaputri, Y. E. (2016). Media yang Digunakan pada Kultur Sel. Farmaka, 14(3), 27-36.

DOI: 10.23917/bioeksperimen.v7i2.11426

Article Metrics

Abstract view(s): 924 time(s)
PDF (Bahasa Indonesia): 525 time(s)

Refbacks

  • There are currently no refbacks.