Email this article Aktivitas Antihiperurisemia Ekstrak Etanol Daun Afrika (Vernonia amygdalina Delile.) pada Mencit Putih Galur Swiss Webster yang Diinduksi Melinjo dan Hati Ayam
Doni Anshar Nuari(1*), Hesti Renggana(2), Cindra Tri Yuniar(3), Mentari Novitasari(4), Asholeha Lulu(5)(1) Prodi Farmasi Fakultas MIPA Universitas Garut, Jl. Jati No.42B, Tarogong, Garut, Indonesia
(2) Prodi Farmasi Fakultas MIPA Universitas Garut, Jl. Jati No.42B, Tarogong, Garut, Indonesia
(3) Sekolah Farmasi Institut Teknologi Bandung, Jl. Ganesha No.10, Bandung, Indonesia
(4) Prodi Farmasi Fakultas MIPA Universitas Garut, Jl. Jati No.42B, Tarogong, Garut, Indonesia
(5) Prodi Farmasi Fakultas MIPA Universitas Garut, Jl. Jati No.42B, Tarogong, Garut, Indonesia
(*) Corresponding Author
Abstract
Hyperuricemic is a condition in which uric acid level in blood exceeds its normal level. Empirically, African leaves (Vernonia amygdalina Delile.) were used to treat diabetic, hypercholesterolemic, fever, hypertension and hyperuricemic by drinking boiled water of African leaves. This in vivo study was performed to determine antihyperuricemic activity of ethanolic extract of African leaves on male mice using the POCT (Point of Care Testing) method. Suspension combination of melinjo and chicken liver juice were used as hyperuricemic inductor. It is given orally for consecutive 9 days and the uric acid level were tested on day 1, 3, 6, and 9. The results showed that the ethanolic extract of African leaves (Vernonia amygdalina Delile.) at dosage 100, 200, and 400 mg/KgBW had a significantly different antihyperuricemic activity against negative control.
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Alara, O.R., Abdurahman, N.H. and Olalere, O.A., 2020. Ethanolic extraction of flavonoids, phenolics and antioxidant from Vernonia amygdalina leaf using two-level factorial design. Journal of King Saud University–Science, 32(1), pp.7–16.
Becker, M.A., Patrick, F.D., Choi, H.K., Dalbeth, N., Storgard, C., Cravets, M. and Scott, B., 2015. An Open-label, 6-month study of allopurinol safety in gout: The LASSO study. Seminars in Arthritis and Rheumatism, 45(2), pp.174-183.
Chen, Y.C., Huang, C.C., Tsai, K.C., Huang, W.J., Huang, W.C., Hsu, Y.C. and Hsu, F.L., 2014. Evaluation of the antihyperuricemic activity of phytochemicals from Davalia farmosana by enzyme Assay and hyperurecemic mice model. Hindawi Publishing Corporation Evidence-Based Complementary and Alternative Medicine, vol 2014, http://dx.doi.org/10.1155/2014/873607
Departemen Kesehatan Republik Indonesia, 2000. Parameter standar umum ekstrak tumbuhan obat cetakan pertama (pp.13-32). Jakarta.
Djohar, M. and Paramitha, R., 2015. Efektivitas rebusan daun salam (Syzygium polyanthum) terhadap penurunan kadar asam urat dalam darah mencit putih jantan. Pharmacy, 12(2), pp.176–185.
Kementerian Kesehatan Republik Indonesia. 2018. Hasil Utama Riskesdas 2018.
Kristiani, R. D., Rahayu, D. and Subarnas, A., 2013. Aktivitas antihiperurisemia ekstrak etanol daun pakis tangkur (Polypodium feei) pada mencit jantan. New Bionatura, 15(3), pp.174–177.
Kusumayanti, G.A.D., Wiardani, N.K. and Sugiani, P.P.S., 2014. Diet mencegah dan mengatasi gangguan asam urat. Jurnal Ilmu Gizi, 5(1), pp.69-78.
Muhtadi, Suhendi, A., Wahyuningtyas, N., and Sutrisna, E.M., 2014. Uji praklinik antihiperurisemia secara in vivo pada mencit putih jantan galur balb-c dari ekstrak daun salam (Syzigium polyanthum walp) dan daun belimbing wuluh (Averrhoa bilimbi l.). Biomedika vol 6(1); 17–23.
Pawestri, U., 2018. Aktivitas antihiperurisemia ekstrak etanol daun kesum (Polygonum minus Huds.) pada mencit jantan galur swiss webster. Skripsi S1 Prodi Farmasi Universitas Garut.
Rahmi, E.P., Kumolosasi, E., Jalil J., Husain, K., Buang, F., Razak, A.F.A. and Jamal, J.A., 2020. Anti-hyperuricemic and anti-inflammatory effects of Marantodes pumilum as potential treatment for gout. Front. Pharmacol, 11. doi: 10.3389/fphar.2020.00289
Rakanita, Y., Hastuti, L., Tandi, J. and Mulayani, S., 2017. Efektivitas antihiperurisemia ekstrak etanol daun seledri (eeds) pada tikus induksi kalium oksonat. Journal of Tropical Pharmacy and Chemistry, 4(1), pp.1-6.
Sinaga, A. F., Bodhi, W. and Lolo, W.A., 2014. Uji efek ekstrak etanol daun salam (Syzygium polyanthum (Wight.) Walp) terhadap penurunan kadar asam urat tikus putih diinduksi potasium oksonat. Pharmacon Jurnal Ilmiah Farmasi, 3(2), pp.141–145.
Sukrama, I.D.M., 2015. Xanthine oxydase inhibition of kombucha tea in hyperuricemia induced wistar rat: decrease of uric acid, malondialdehyde, and 8-hydroxy-2’-deoxyguanosine. Bali Medical Journal, 4(1), pp.32–36.
Sumarmin, R., Yuniarti, E., and Zulino, G., 2017. Uji in vivo ekstrak buah mahkota dewa (Phaleria macrocarpa (Scheff.) Boerl.) terhadap kadar asam urat pada mencit jantan (Mus musculus L.). Journal Biosains, 1(2), pp.57–61.
Sun, C., Wu, Z., Wang, Z. and Zhang, H., 2015. Effect of ethanol/water solvent on phenolic profiles and antioxidant properties of beijing propolis extracts. Hindawi Publishing Corporation Evidence-Based Complementary and Alternative Medicine, Vol 2015 http://dx.doi.org/10.1155/2015/595393
Tarigan, I. M., Bahri, S. and Saragih, A., 2012. Aktivitas antihiperurisemia ekstrak etanol herba suruhan (Peperomia pellucida (L.) Kunth) pada mencit jantan. Journal of Pharmaceutics and Pharmacology, 1(1), pp.37–43.
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