Aktivitas Sitotoksik dan Antiproliferasi Fraksi n-Heksan Biji Al-pukat (Percea americana Mill.) Terhadap sel T47D

Juwita Rahmawati(1), Maryati Maryati(2*)

(1) Fakultas Farmasi, Universitas Muhammadiyah Surakarta ,Jl. Ahmad Yani, Pabelan, Kartasura, Surakarta 57162
(2) Fakultas Farmasi, Universitas Muhammadiyah Surakarta ,Jl. Ahmad Yani, Pabelan, Kartasura, Surakarta 57162
(*) Corresponding Author


Avocado plants are widely used as traditional medicine in Indonesia. This research aimed to determine the cytotoxic and antiproliferation activity of avocado seed extract and fractions on the T47D breast cancer cell line. Extraction was done by maceration method using 96% ethanol, then fractionation was done by liquid-liquid partition using n-hexane, chloroform, and ethyl acetate. Cytotoxic and antiproliferation tests were done by the MTT method. Apoptosis activity was investigated by the double staining method, using acridine orange-propidium iodide as a staining reagent. Results showed that the n-hexane fraction of avocado seed had a cytotoxic activity with IC50 27.9 µg/mL. N-hexane fraction of avocado seed inhibited proliferation and induced apoptosis of T47D cell line.


Percea americana Mill; breast cancer; MTT; cytotoxic; Apoptosis

Full Text:



Alenzi, F. 2014. Links between apoptosis, proliferation and the cell cycle, British Journal Of Biomedical Science, 61(2). https://doi.org/10.1080/09674845.2004.11732652

Ammerman, N. C., Beier-Sexton, M., Azad, A. F. 2009. Growth and Maintenance of Vero Cell Lines Nicole. NIH, 1–10. https://doi.org/10.1002/9780471729259.mca04es11.Growth

Bjelaković, G., Nagorni, A., Bjelaković, M., Stamenković, I., Arsic, R., Katic, V. 2005. Apoptosis: Programmed Cell Death And Its Clinical Implications, Medicines and Biology, 12(1), 6–11.

CCRC. 2009a. Prosedur Tetap Pengamatan Apoptosis Dengan Metode Double Stainning (CCRC-03-011-01).

CCRC. 2009b. Prosedur Tetap Uji Pengamatan Proliferasi Sel (Dubling Time) (CCRC-03-015-01).

CCRC. 2012. Prosedur Tetap Uji Sitotoksik Metode MTT (CCRC-03-010-01).

Christiansen, B. A., Bhatti, S., Goudarzi, R., Emami, S. 2015. Management of Osteoarthritis with Avocado / Soybean Unsaponifiables, Sage, 6(1), 30–44. https://doi.org/10.1177/1947603514554992

D’Ambrosio, S. M., Han, C., Pan, L., Kinghorn, A. D., Ding, H. 2011. Aliphatic acetogenin constituents of avocado fruits inhibit human oral cancer cell proliferation by targeting the EGFR/RAS/ RAF/MEK/ERK1/2 pathway, National Institute Of Health, 10; 409(3), 1–11. https://doi.org/10.1016/j.bbrc.2011.05.027.Aliphatic

Dabas, D., Elias, R. J., Ziegler, G. R., Lambert, J. D. 2019. In Vitro Antioxidant and Cancer Inhibitory Activity of a Colored Avocado Seed Extract. International Journal of Food Science, 2019(2017). https://doi.org/10.1155/2019/6509421

Désiré, P., Dzeufiet, D., Mogueo, A., Bilanda, D. C., Aboubakar, B. O., Tédong, L., Dimo, T., Kamtchouing, P. 2014. Antihypertensive potential of the aqueous extract which combine leaf of Persea americana Mill . ( Lauraceae ), stems and leaf of Cymbopogon citratus ( D . C ) Stapf . ( Poaceae ), fruits of Citrus medical L . ( Rutaceae ) as well as honey in ethanol and s. Complementary and Alternative Medicine, 14:507, 1–12.

Dipiro, J. T., Wells, B. G., Schwingammer, T. L., & Dipiro, V. C. 2012. Pharmacotherapy Handbook (9th ed.). Mc Graw Hill Education.

Ezejiofor, A. N., Okorie, A., & Orisakwe, E. 2013. Hypoglycaemic and Tissue-Protective Effects of the Aqueous Extract of Persea Americana Seeds on Alloxan-Induced Albino Rats. Malays J Med Sci, 20(5), 31–39.

Foglieni, C., Meoni, C., & Davalli, A. M. 2001. Fluorescent dyes for cell viability: An application on prefixed conditions, Springer, 115(April 2001), 223–229. https://doi.org/10.1007/s004180100249

Lara-Márquez, M., Báez-Magaña, M., Raymundo-Ramos, C., Spagnuolo, P. A., Macías-Rodríguez, L., Salgado-Garciglia, R., Ochoa-Zarzosa, A., López-Meza, J. E. (2020). Lipid-rich extract from Mexican avocado (Persea americana var. drymifolia) induces apoptosis and modulates the inflammatory response in Caco-2 human colon cancer cells. Journal of Functional Foods, 64(October 2019), 103658. https://doi.org/10.1016/j.jff.2019.103658

Mahmoed, M. Y., dan Rezq, A. A. 2013. Hepatoprotective Effect of Avocado Fruits Against Carbon Tetrachloride-Induced Liver Damage in Male Rats. World Applied Sciences Journal, 21(10), 1445–1452. https://doi.org/10.5829/idosi.wasj.2013.21.10.72160

Malumbres, M., & Barbacid, M. 2007. Cell cycle kinases in cancer. Elsevier, 17, 60–65. https://doi.org/10.1016/j.gde.2006.12.008

Mardiyaningsih, A., dan Ismiyati, N. 2014. Cytotoxic Activity Of Ethanolic Extract Of Persea americana Mill. Leaves On HeLa Cervical Cancer Cell. Traditional Medicine Journal,Raditional Medicine Journal, 19, 24–28.

Maryati, M., Sutrisna, E., Saifudin, A., Kusumaningrum, I., Abu bakar, M. . 2019. Extract of ceplukan ( Physalis angulata L .) inhibited proliferation and induced apoptosis in myeloma cell line. Food Research, 3(December), 755–760. https://doi.org/10.26656/fr.2017.3(6).169

Mascotti, K., Mccullough, J., & Burger, S. 2000. HPC viability measurement: Trypan blue versus acridine orange and propidium iodide. Transfusion, 40, 693–696. https://doi.org/10.1046/j.1537-2995.2000.40060693.x

Mehrara, E., Forssell-aronsson, E., Bernhardt, P. 2007. Specific Growth Rate versus Doubling Time for Quantitative Characterization Specific Growth Rate versus Doubling Time for Quantitative Characterization of Tumor Growth Rate, May 2014. https://doi.org/10.1158/0008-5472.CAN-06-3822

Melgar, B., Inês, M., Ciric, A., Sokovic, M., Garcia-castello, E. M., Rodriguez-lopez, A. D., Barros, L., Ferreira, I. C. R. F. 2018. Bioactive characterization of Persea americana Mill . by-products : A rich source of inherent antioxidants, Industrial Crops & Products, 111(2017), 212–218. https://doi.org/10.1016/j.indcrop.2017.10.024

Momenimovahed, Z., dan Salehiniya, H. 2019. Epidemiological characteristics of and risk factors for breast cancer in the world. Dovepress, 11, 151–164.

Morcuende, D., Kylli, P., Est, M. 2011. Avocado ( Persea americana Mill .) Phenolics , In Vitro Antioxidant and Antimicrobial Activities , and Inhibition of Lipid and Protein Oxidation in Porcine Patties. Journal of Agricultural and Food Chemistry, 59, 5625–5635.

Nur, A., Abubakar, F., Achmadi, S. S., Suparto, I. H. 2017. Triterpenoid of avocado (Persea americana) seed and its cytotoxic activity toward breast MCF-7 and liver HepG2 cancer cells. Asian Pacific Journal of Tropical Biomedicine, 7(5), 397–400. https://doi.org/10.1016/j.apjtb.2017.01.010

Paul, R., Kulkarni, P., Ganesh, N. 2011. Avocado fruit (Persea americana Mill) exhibits chemo-protective potentiality against cyclophosphamide induced genotoxicity in human lymphocyte culture. Journal of Experimental Therapeutics and Oncology, 9(3), 221–230.

Sutejo, I. R., Putri, H., Meiyanto, E. 2016. Selektivitas Ekstrak Etanolik Buah Makassar (Brucea javanica ) pada Kanker Payudara Metastasis secara In Vitro.The Selectivity of Ethanolic Extract of Buah Makassar ( Brucea javanica ) on In Vitro Study of Metastatic Breast Cancer, Journal of Agromedicine and Medical Sciences, 2(1), 1–5.

Velez-pardo, C. 2014. Pro-apoptotic effect of Persea americana var. Hass (avocado) on Jurkat lymphoblastic leukemia cells. Informa Healthcare, 52(4), 458–465. https://doi.org/10.3109/13880209.2013.842599

Vo, T. S., Le, P. U., Ngo, D. H. 2019. Free radical scavenging and anti-proliferative activities of avocado (Persea americana Mill.) seed extract. 9(3), 91–97. https://doi.org/10.4103/2221-1691.254602

Wati, E. M., Puspaningtyas, A. R., Pangaribowo, D. A. 2016. Uji Sitotoksisitas dan Proliferasi Senyawa 1- ( 4-nitrobenzoiloksi- metil ) -5-fluorourasil terhadap Sel Kanker Payudara MCF-7 methyl ) -5-fluorouracil ) on Breast Cancer Cells MCF-7). Pustaka Kesehatan, 4(3), 484–488.

WHO. 2018. Latest global cancer data : Cancer burden rises to 18.1 million new cases and 9 . 6 million cancer deaths in 2018 Latest global cancer data : Cancer burden rises to 18.1 million new cases and 9.6 million cancer deaths in 2018. International Agency for Research on Cancer, September, 13–15.

WHO. 2019. Global Cancer Statistics: Indonesia Fact Sheets, International Agency for Research on Cancer, 256, 1–2.

Widiastuti, Y., Pratiwi, R., Riyanto, S., Wahyuono, S. 2018. Persea americana Mill . seed extract on MCF-7 cancer cell line. Indonesian Journal of Biotechnology, 23(2), 61–67. https://doi.org/10.22146/ijbiotech.32141

Wong, R. S. 2011. Apoptosis in cancer; from pathogenesis to treatment. Journal of Experimental & Clinical Cancer Research, 30:87(2), 1–14.

Article Metrics

Abstract view(s): 696 time(s)
PDF: 1121 time(s)


  • There are currently no refbacks.