Uji Aktivitas Antikolesterol Kombinasi Ekstrak Teh Oolong, Buncis Dan Kayu Manis

Ahmad Fauzi(1*), Tanti Azizah Sujono(2), Azis Saifudin(3), Arifah Sri Wahyuni(4)

(1) Universitas Muhammadiyah Surakarta
(2) Universitas Muhammadiyah Surakarta
(3) Universitas Muhammadiyah Surakarta
(4) Universitas Muhammadiyah Surakarta
(*) Corresponding Author


Hyperlipidemia has an important role in the occurrence of cardiovascular, cerebrovascular, and peripheral vascular diseases. High cholesterol and LDL levels in the blood are at risk of causing various diseases such as atherosclerosis, pancreatic dysfunction and kidney disorders. Meanwhile, high triglyceride levels can cause impaired liver, pancreas and kidney function. Green bean, oolong tea and cinnamon are plants that can lower cholesterol levels. Oolong tea contains catechins, Green Bean contain phytosterols and cinnamon contains cinnamaldehyde. Determination of secondary metabolite levels of extracts was carried out using the Liquid Chromatography – Mass Spectroscopy (LC-MS) method. The activity test was carried out with 30 Wistar white rats which were divided into 6 test groups. The normal control group was treated with CMC-Na without feeding hypercholesterolemia, the positive control group was treated with simvastatin 0.4 mg/KgBW, the negative control group was treated with CMC-Na, the test groups 1, 2 and 3 were treated with cinnamon extract, oolong tea and green bean, respectively (150:50:50 mg/KgBW), (50:150:50 mg/KgBW) and (50:50:150 mg/KgBW). The detected metabolite content was cinnamaldehyde in cinnamon up to 10%, catechins in oolong tea up to 12% and stigmasterol in beans as much as 3.4%.The results obtained by test group 1 and test group 2 can reduce cholesterol and triglyceride levels (p<0.05). All test groups had no significant effect on HDL and LDL levels.


Oolong tea, green beans, cinnamon, cholesterol, LC-MS

Full Text:



Abdelgadir A., Hassan H., Eltaher A., Mohammed GA K., Mohammed AA L., TB H., TH Aboalbashar, TH Aalim, AM A. dan AK M., 2020, Hypolipidemic Effect of Cinnamon (Cinnamomum zeylanicum) Bark Ethanolic Extract on Triton X-100 induced Hyperlipidemia in Albino Rats, Medicinal & Aromatic Plants, 9 (3), 531-537.

Abeysekera W.P.K.M., Arachchige S.P.G. dan Ratnasooriya W.D., 2017, Bark Extracts of Ceylon Cinnamon Possess Antilipidemic Activities and Bind Bile Acids In Vitro, Evidence-based Complementary and Alternative Medicine, 2017 (3), 139–148.

Adelina R., 2018, Mekanisme Katekin Sebagai Obat Antidislipidemia (Uji In Silico), Buletin Penelitian Kesehatan, 46 (3), 147–154.

Al-Zahrani J., Shubair M.M., Al-Ghamdi S., Alrasheed A.A., Alduraywish A.A., Alreshidi F.S., Alshahrani S.M., Alsalamah M., Al-Khateeb B.F., Ashathri A.I., El-Metwally A. dan Aldossari K.K., 2021, The prevalence of hypercholesterolemia and associated risk factors in Al-Kharj population, Saudi Arabia: a cross-sectional survey, BMC Cardiovascular Disorders, 21 (1), 1–8.

Alam S., Siddiq A. dan Mohtashhemul Hasan M., 2016, Hypolipidemic Effect of Phaseolus Vulgaris L . At Various Doses and Its Impact on Cardiovascluar, World Journal of Pharmacy and Pharmaceutical Sciences, 5 (11), 163–174.

Batta A.K., Xu G., Honda A., Miyazaki T. dan Salen G., 2006, Stigmasterol reduces plasma cholesterol levels and inhibits hepatic synthesis and intestinal absorption in the rat, Metabolism: Clinical and Experimental, 55 (3), 292–299.

Chávez-Santoscoy R.A., Tovar A.R., Serna-Saldivar S.O., Torres N. dan Gutiérrez-Uribe J.A., 2014, Conjugated and free sterols from black bean (Phaseolus vulgaris L.) seed coats as cholesterol micelle disruptors and their effect on lipid metabolism and cholesterol transport in rat primary hepatocytes, Genes and Nutrition, 9 (1), 367.

Cherng S., 2017, HMG-CoA reductase ( 3-hydroxy-3-methyl-glutaryl-CoA reductase )( HMGR ), The Journal of American Science, 4(3), January 2008, 1–4.

Deng X., Hou Y., Zhou H., Li Y., Xue Z., Xue X., Huang G., Huang K., He X. dan Xu W., 2021, Hypolipidemic, anti-inflammatory, and anti-atherosclerotic effects of tea before and after microbial fermentation, Food Science and Nutrition, 9 (2), 1160–1170.

Fauziah S., Syech R. dan Sugianto, 2019, Pengujian Kualitas Minyak Goreng Kemasan, Curah yang Beredar di Daerah Panam Pekanbaru dan Minyak Goreng Jelantah Berdasarkan Sifat Fisika, Universitas Riau, 53 (9), 1–6.

Friedewald W.T., Levy R.I. dan Fredrickson D.S., 1972, Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge., Clinical chemistry, 18 (6), 499–502.

Goldberg I.J., Eckel R.H. dan Abumrad N.A., 2009, Regulation of fatty acid uptake into tissues: Lipoprotein lipase- And CD36-mediated pathways, Journal of Lipid Research, 50 (Suppl.)

Górska-Warsewicz H., Rejman K., Laskowski W. dan Czeczotko M., 2019, Butter, margarine, vegetable oils, and olive oil in the average polish diet, Nutrients, 11 (12), 1–15.

Haddouchi F., 2013, Chemical composition and antimicrobial activity of the essential oils from Ruta species growing in Algeria, Journal Food Chemistry, 141 (October), 253–258.

Van Herck H., Baumans V., Brandt C.J.W.M., Boere H.A.G., Hesp A.P.M., Van Lith H.A., Schurink M. dan Beynen A.C., 2001, Blood sampling from the retro-orbital plexus, the saphenous vein and the tail vein in rats: Comparative effects on selected behavioural and blood variables, Laboratory Animals, 35 (2), 131–139.

Idacahyati K., Sukmawan Y.P., Yanti N., Wulandari W.T., Gustaman F. dan Indra, 2021, In vivo activity of phaseolus vulgaris as an anti-hypercholesterolemic, Pharmacy Education, 21 (2), 184–188.

Jiang K., Gachumi G., Poudel A., Shurmer B., Bashi Z. dan El-Aneed A., 2019, The Establishment of Tandem Mass Spectrometric Fingerprints of Phytosterols and Tocopherols and the Development of Targeted Profiling Strategies in Vegetable Oils, Journal of the American Society for Mass Spectrometry, 30 (9), 1700–1712.

Kaur J., 2014, Use of friedewald equation for dyslipidemia in metabolic syndrome, International Journal of Medicine, 2 (1), 36.

Khan S.H., Niazi N.K., Sobia F., Fazal N., Manzoor S.M. dan Nadeem A., 2019, Friedewald’s equation for calculating LDL-cholesterol: Is it the time to say “goodbye” and adopt direct LDL cholesterol methods?, Pakistan Journal of Medical Sciences, 35 (2), 388–393.

Kim D., Park J.B., Choi W.K., Lee S.J., Lim I. dan Bae S.K., 2016, Simultaneous determination of β-sitosterol, campesterol, and stigmasterol in rat plasma by using LC–APCI-MS/MS: Application in a pharmacokinetic study of a titrated extract of the unsaponifiable fraction of Zea mays L., Journal of Separation Science, 39 (21), 4060–4070.

Laufs U., Parhofer K.G., Ginsberg H.N. dan Hegele R.A., 2020, Clinical review on triglycerides, European Heart Journal, 41 (1), 99–109.

Li J., Liu T., Wang L., Guo X., Xu T., Wu L., Qin L. dan Sun W., 2012, Antihyperglycemic and antihyperlipidemic action of cinnamaldehyde in C57blks/j Db/db mice, Journal of Traditional Chinese Medicine, 32 (3), 446–452.

Nanang Y., Sulistyowati I., Finolawati A., Elya B. dan Rani S., 2021, HMG-CoA Reductase Inhibitory Activity Of Extract And Catechin Isolate From Uncaria Gambir As A Treatment For Hypercholesterolemia, Journal Of Southwest Jiaotong University, 56 (6), 37–41.

Neto J.G.O., Boechat S.K., Romão J.S., Pazos-Moura C.C. dan Oliveira K.J., 2020, Treatment with cinnamaldehyde reduces the visceral adiposity and regulates lipid metabolism, autophagy and endoplasmic reticulum stress in the liver of a rat model of early obesity, The Journal of nutritional biochemistry, 77, 108

Pishdad S., Nadjarzadeh A., Abargouei A.S., Nazari E.K. dan Papoli M., 2018, Effect of cumin and cinnamon on lipid profile in middle-aged women with dyslipidemia: A double blind, randomized controlled clinical trial, Progress in Nutrition, 20 (8), 232–237.

Rodríguez L., Mendez D., Montecino H., Carrasco B., Arevalo B., Palomo I. dan Fuentes E., 2022, Role of Phaseolus vulgaris L. in the Prevention of Cardiovascular Diseases-Cardioprotective Potential of Bioactive Compounds, Plants, 11 (2),186.

Sae-tan S., 2016, Systematic Review: Hypolipidemic Activity of Oolong Tea Polymerized Polyphenols, Journal of Health Research, 30 (6), 451–459.

Samavat H., Newman A., Wang R., Yuan J.-M., Wu A.H. dan Kurzer M.S., 2016, Effect of green tea cathecin extract on serum lipid in post-menepousal woman, Am J Clin Nutr 2016;104,1671–82.

Sonsok S., Sarakarn P. and Sanchaisuriya P., 2020, Comparison of three equations for estimating low-density lipoprotein-cholesterol in the rural northeastern region of Thailand, Journal of Clinical Laboratory Analysis, 34 (10),1-8.

Tilokwattanothai A., Saenphet K. dan Saenphet S., 2017, Hypoglycemic and hypolipidemic properties of herbal tea on Wistar rat, Chiang Mai Veterinary Journal 2017; 15(1,25-35

Wahjuni S., Rustini N. dan Yuliantari P., 2016, Pemberian ekstrak etanol buah buncis (Phaseolus vulgaris L.) untuk menurunkan kolesterol total, low density lipoprotein (LDL) dan meningkatkan high density, Jurnal Kimia 10 (1), 2016, 103-109

Zhu Z., Li J., Gao X., Amponsem E., Kang L., Hu L., Zhang B. dan Chang Y., 2012, Journal of Pharmaceutical and Biomedical Analysis Simultaneous determination of stilbenes , phenolic acids , flavonoids and anthraquinones in Radix polygoni multiflori by LC – MS / MS, Journal of Pharmaceutical and Biomedical Analysis, 62, 162–166.

Zhyvotovska A., Yusupov D. dan I. McFarlane S., 2019, Introductory Chapter: Overview of Lipoprotein Metabolism, Dyslipidemia, 40(8), 0–7.

Article Metrics

Abstract view(s): 819 time(s)
PDF: 863 time(s)


  • There are currently no refbacks.