Cytotoxic Activities of Ethanol Extract, Nonpolar Semipolar, and Polar Fractions of Dioscorea esculenta L.) on MCF7 Cancer Cell

H Haryoto(1*)

(1) Fakultas Farmasi Universitas Muhammadiyah SUrakarta
(*) Corresponding Author

Abstract

One of the disease that have a high contribution to the death of women in the world is breast cancer. It leads to the research and exploration to find its cure. This study is conducted to know the anti-cancer activity onethanol extract, nonpolar, semipolar, and polar fractions of lesser yam (Dioscorea esculenta L.) herbs. It also aims to find out the content of compound on semipolar fraction Dioscorea esculenta L. herbs. The extraction was carried out using maceration technique with 96% ethanol as solvent. Then, the fractination was done using vacuum liquid chromatography method with n-hexane and acetone as mobile phase with the ratio of 9:1, 8:2, 7:3, 6:4, and 5:5. After that, the phytochemical test was undertaken using the thin layer chromatographywith n-hexane and acetone as mobile phase with proportion 7:3. Meanwhile, the cytotoxic test was conducted using MTT assay method with IC50calculation was counted using linear regression between the log concentration and the percentage of living cells. The semipolar fraction of Dioscorea esculenta L. herbs has IC50value of 632,42 µg/mL, whereas the ethanol extract, nonpolar and polar fractions have the IC50 value > 1000 µg/mL. Furthermore, the compound of the semipolar fraction of Dioscorea esculenta L. herbs are saponins, terpenoids, alkaloids, and phenolics.

Keywords

Lesser yam herbs, Dioscorea esculenta L., cytotoxic, MCF7, MTT assay.;

Full Text:

PDF

References

Bézivin C., Tomasi S., Lohézic-Le Dévéhat F. and Boustie J., 2003, Cytotoxic Activity of Some Lichen Extracts on Murine and Human Cancer Cell Lines, Phytomedicine, 10 (6–7), 499–503.

Chiang C., Way T., Tsai S. and Lin J., 2007, Diosgenin, A Naturally Occurring Steroid, Suppresses Fatty Acid Synthase Expression in HER2-Overexpressing Breast Cancer Cells Through Modulating Akt, mTOR and JNK Phosphorylation, FEBS Letters, 581, 5735–5742.

Haryoto, 2013, Teknik Uji Hayati Untuk Pengembangan Obat (TUHPO), Fairuz Media, Surakarta.

Hermawan A. and Meiyanto E., 2010, Hesperidin Meningkatkan Efek Sitotoksik Doxorubicin pada Sel MCF-7, Majalah Farmasi Indonesia, 21 (1), 8–17.

Mar’atirrosyidah R. and Estiasih T., 2015, Aktivitas Antioksidan Senyawa Bioaktif Umbi Lokal Inferior : Kajian Pustaka, Jurnal Pangan dan Agroindustri, 3 (2), 594–601.

Marie-Aleth L.-D., 2005, Bioactive Saponins with Cancer Related and Immunomodulatory Activity: Recent Developments, Atta-ur-Rahman (Ed.) Studies in Natural Products Chemistry, 32, 209–246.

Meiyanto E., Fitriasari A. and Hermawan A., 2011, The Improvement of Doxorubicin Activity on Breast Cancer Cell Lines by Tangeretin Through Cell Cycle Modulation, Orient Pharm Exp Med, 183–190.

Meiyanto E., Supardjan, Da’i M. and Agustina D., 2006, Efek Antiproliferatif Pentagamavunon-0 terhadap Sel Kanker Payudara T47D, Jurnal Kedokteran Yarsi, 14 (1), 11–15.

Muzaimah S., Farina A.H., Yaacob W.A., Ahmad I.B. and Din L.B., 2010, A Survey on Phytochemical and Bioactivity of Plant Extracts From Malaysian Forest Reserves, Journal of Medicinal Plants Research, 4 (February), 203–210.

Olayemi J.O. and Ajaiyeoba E.O., 2007, Anti-inflammatory Studies of Yam (Dioscorea esculenta) Extract on Wistar Rats, African Journal of Biotechnology, 6 (August), 1913–1915. Terdapat di: http://www.academicjournals.org/AJB.

Riss T.L., Moravec R.A., Niles A.L., Duellman S., Benink H.A., Worzella T.J. and Minor L., 2013, Cell Viability Assays, Assay Guidance Manual [Internet], 114 (8), 785–796. Terdapat di: http://www.ncbi.nlm.nih.gov/books/NBK144065/?report=reader%5Cnhttp://www.ncbi.nlm.nih.gov/pubmed/23805433.

Saifudin A., 2014, Senyawa Alam Metabolit Sekunder Teori, Konsep dan Teknik Pemurnian, Deepublish, Yogyakarta.

Sautour M., 2007, The Dioscorea Genus: A Review of Bioactive Steroid Saponins, J Nat Med, 61, 91–101.

Soetoko D.F. and Sumarno, 2012, Anticancer Effect and Cell Cycle Analysis of Ethanolic Extract of Gembili (Dioscorea esculenta L. Burkill) on T47D Breast Cancer Cell Line, International Conference: Research and Application on Traditional Complementary and Alternative Medicine in Health Care (TCAM), 22–23.

Wagner H. and Bladt S., 2001, Plant Drug Analysis, 2nd ed., Springer, Munchen.

Wagner M.A.L., 2000, Bioactive Saponins From Plants: An Update, Atta-ur-Rahman (Ed.) Studies in Natural Products Chemistry, 21, 633–687.

DOI: 10.23917/jnhm.v2i1.7853

Article Metrics

Abstract view(s): 321 time(s)
PDF: 397 time(s)

Refbacks

  • There are currently no refbacks.