Uji Cemaran Mikroba dan Kapang Khamir Ekstrak Air Daun Muntingia calabura L. (Kersen)

Aulia Nur Rahmawati(1*), Dwi Saryanti(2), Fryda Nurita Sari(3), Irma Yovita Turnip(4)

(1) Sekolah Tinggi Ilmu Kesehatan Nasional
(2) Sekolah Tinggi Ilmu Kesehatan Nasional
(3) Sekolah Tinggi Ilmu Kesehatan Nasional
(4) Sekolah Tinggi Ilmu Kesehatan Nasional
(*) Corresponding Author

Abstract

Kersen (Mutingia calabura L.) is a plant that can be used as a raw material for medicine because it has many medicinal benefits. Kersen as a medicinal raw material must pass a quality parameter testing process to be safe to use. One of the extract quality parameters is the total plate count for microbial (ALT) and yeast contamination (AKK). This study aims to evaluate the suitability of microbial and yeast contamination in the water extract of Kersen leaves to the applicable regulations. Extraction of Kersen leaves used maceration method with water solvent for 3 days with a ratio of 1:10. The results obtained from the water extract of Kersen leaves are organoleptically in the form of thick extract, dark brown in color, sour taste, bitter, and has a distinctive odor. The ALT value in the aqueous extract of cherry leaves was 8.4 x 10⁴ colonies/g. The AKK value in the aqueous extract of cherry leaves was 102 colonies/g which was reported as an approximate AKK value. The ALT and AKK values in the water extract of Kersen leaves meet the safety and quality requirements of traditional medicine.

Keywords

Muntingia calabura L., kersen leaf water extract, total plate count, microbial contamination, yeast contamination

Full Text:

PDF

References

Alviani, N., & Fitri, K., 2018, Formulasi Sediaan Krim Anti Jerawat Ekstrak Etanol Daun Kersen (Muntingia calabura L.). Jurnal Dunia Farmasi , 3(1), 24–31.

American Herbal Products Association, 2003, Standardization of Botanical Products. American Herbal Products Association.

Anggraini, D. I., & Kusuma, E. W., 2020, Uji Cemaran pada Ekstrak Etanol Tempe Koro Benguk (Mucuna pruriens L.) Sebagai Obat Antidiabetes Terstandar. Jurnal Ilmiah Cendikia Eksakta, 5(1), 1–11.

Arsa, A. K., & Achmad, Z., 2020, Ekstraksi Minyak Atsiri dari Rimpang Temu Ireng (Curcuma aeruginosa Roxb) dengan Pelarut Etanol Dan N-Heksana . Jurnal Teknologi Technoscientia, 13(1), 83–94.

Balan, T., Sani, M. H. M., Ahmad, S. H. M., Suppaiah, V., Mohtarrudin, N., & Zakaria, Z. A., 2015, Antioxidant and anti-inflammatory activities contribute to the prophylactic effect of semi-purified fractions obtained from the crude methanol extract of Muntingia calabura leaves against gastric ulceration in rats . Journal of Ethnopharmacol, 164, 1–15.

Departemen Kesehatan Republik Indonesia, 2000, Parameter Standar Mutu Ekstrak Tanaman Obat . Departemen Kesehatan Republik Indonesia.

Einhorn-Stoll, U., 2018, Pectin-water interactions in foods – From powder to gel. Food Hydrocolloids, 78, 109–119.

Haerani, A., 2020, Potensi Tanaman Kersen (Muntingia calabura L.) sebagai Kosmetik: Review. Jurnal Kesehatan Rajawali, 10(2), 61–67.

Hamid, M. K., 2017, Aktivitas Antipenuaan Ekstrak Air Daun Kersen (Muntingia calabura) Terhadap Jumlah Fibroblas Kulit . Universitas Islam Indonesia Yogyakarta.

Korompis, F. C. , Yamlean, P. V. Y., & Lolo, W. A., 2020, Formulasi dan Uji Efektivitas Antibakteri Sediaan Sabun Cair Ekstrak Etanol Daun Kersen (Muntingia calabura L.) Terhadap Bakteri Staphlococcous epidermidis. Jurnal Pharmacon, 9(1), 30–37.

Lailiyah, M., Restyana, A., & Setyarti, O. B., 2019, Formulasi Facial Wash Gel Ekstrak Etanol Daun Kersen (Muntingia calabura) terhadap Bakteri Propionibacterium acnes secara in Vitro. Jurnal Inovasi Farmasi Indonesia, 24–32.

Marjoni, M. R., Afrinaldi, A., & Novita, A. D., 2015, Kandungan Total Fenol dan Aktivitas Antioksidan Ekstrak Air Daun Kersen (Mutingia calabura L.). Jurnal Kedokteran Yarsi, 23(3), 187–196.

Maulana, S., 2015, Ekstraksi dan Karakterisasi Pektin dari Limbah Kulit Pisang Uli (Musa paradisiaca L. ABB . UIN Syariff Hidayatullah Jakarta.

Puspitasari, A. D., Mulangsri, D. A. K., & Herlina, H., 2018, Formulasi Krim Tabir Surya Ekstrak Etanol Daun Kersen (Muntingia calabura) untuk Kesehatan Kulit. Media Litbangkes, 28(4), 263–270.

Puspitasari, A. D., & Proyogo, L. S., 2017, Perbandingan Metode Ekstraksi Maserasi dan Sokletasi terhadap Kadar Fenolik Total Ekstrak Etanol Daun Kersen (Muntingia calabura). Jurnal Ilmiah Cendikia Eksakta , 2(1), 1–8.

Rahmawati, A. N., Astirin, O. P., & Pangastuti, A., 2018, Intracellular antioxidant activity of Muntingia calabura leaves methanolic extract. Nusantara Bioscience, 10(3), 210–214.

Rahmawati, A. N., Astirin, O. P., & Pangastuti, A., 2019, The Effect of Muntingia calabura L. Leaves Methanolic Extract in Increasing of Collagen Production. AIP Conference Prosiding , 2094, 1–5.

Saifudin, A., Rahayu, V., & Teruna., 2011, Standarisasi Bahan Obat Alam. Yogyakarta: Graha Ilmu. Graha Ilmu .

Sari, I., Miranda, T., & Sadli, S., 2016, The Cytotoxic Activity Of N-Hexane Extract Of Kersen (Muntingia calabura Linn.) Leaves Using The Brine Shrimp Lethality Test (BSLT) Method. Jurnal Natural , 16(2), 37–44.

Saweng, C. F. I. J., Sudimartini, L. M., & Suartha, I. N., 2020, Uji Cemaran Mikroba pada Daun Mimba (Azadiractha indica A. Juss) Sebagai Standarisasi Bahan Obat Herbal. . . Indonesia Medicus Veterinus, 9(2), 270–280.

Senet, M. R. M., Raharja, I. G. M. A. P., Darma, I. K. T., Prastakarini, K. T., Dewi, N. M. A., & Parwata, I. M. O. A., 2018, Penentuan Kandungan Total Flavonoid dan Total Fenol dari Akar Kersen (Muntingia calabura) Serta Aktivitasnya Sebagai Antioksidan. Jurnal Kimia, 12(1), 13–18.

Sulistyoningrum, E., Rosmelia, R., Hamid, M. K., & Nuraini, W. S. T., 2019, Antiaging Effect of Muntingia calabura Leaves Extract in D-galactose-induced Skin Aging Mouse Model. . Journal of Applied Pharmaceutical Science, 9(9), 23–29.

Syahara, S., & Siregar, Y. F., 2019, Skrining Fitokimia Ekstrak Etanol Daun Kersen (Muntingia Calabura). Jurnal Kesehatan Ilmiah Indonesia, 4(2), 121–125.

Syahidan, H. H., & Wardhana, Y. W., 2019, Review Jurnal: Parameter Standarisasi Tanaman Herbal untuk Pengobatan. Farmaka , 17(2), 263–274.

Voigt, T., 1994, Pelajaran Teknologi Farmasi. Gajah Mada University Press.

Zakaria, Z. A., Balan, T., Suppaiah, V., Ahmad, S., & Jamaludin, F., 2014, Mechanism(s) of action involved in the gastroprotective activity of Muntingia calabura. Journal of Ethnopharmacology, 151(3), 1184–1193.

Zakaria, Z. A., Mohamed, A. M., Jamil, N. S. M., Rofiee, M. S., Hussain, M. K., Sulaiman, M. R., Teh, L. K., & Salleh, M. Z., 2011, In Vitro Antiproliferative and Antioxidant Activities of the Extracts of Muntingia calabura Leaves. The American Journal of Chinese Medicine, 39(01), 183–200.

DOI: 10.23917/pharmacon.v19i1.18304

Article Metrics

Abstract view(s): 1558 time(s)
PDF: 6051 time(s)

Refbacks

  • There are currently no refbacks.