Potensi Ekstrak Etanol Herba Apu-Apu (Pistia stratiotes) dan Fraksi-Fraksinya Sebagai Antioksidan Dengan Metode DPPH

Dewi Dianasari(1*), Irawati Firdiyansari(2)

(1) Fakultas Farmasi, Universitas Jember, Jember
(2) Fakultas Farmasi, Universitas Jember, Jember
(*) Corresponding Author

Abstract

Free radicals play an important role in cell damage and degenerative diseases such as coronary heart disease, stroke, premature aging and others. Compounds that can inhibit free radicals are antioxidants. One of the plants that has a high phenolic compound and can be a source of natural antioxidants, apu-apu or Pistia stratiotes. This research was conducted to scientifically prove the antioxidant activity of the apu-apu herbal extract (Pistia stratiotes) and its fractions by the DPPH method. The sample used in the form of all parts apu-apu (apu-apu herb) obtained from the Sumbersari District, Jember Regency. In this study the DPPH method was used with a DPPH concentration of 0.1 mM, incubation time of ethanol extract 40 minutes, fraction of n-hexane 50 minutes, ethyl acetate fraction 45 minutes, ethanol-water fraction 40 minutes,vitamin C 35 minutes and measured at wavelength 517 nm . The results of the average antioxidant activity (IC50) were apu-apu herbal extract 16.675 µg / mL ± 0.239, n-hexane fraction 29.915 µg / mL ± 0.156, ethyl acetate fraction 11.875 µg / mL ± 0.038, ethanol-water fraction 9.090 µg / mL ± 0.156, ethyl acetate fraction 11.875 µg / mL ± 0.038, ethanol-water fraction 9.090 µg / mL ± 0.156, ethyl acetate fraction 11.875 µg / mL ± 0.038 / mL ± 0.122, and vitamin C 3.263 µg / mL ± 0.032. Vitamin C as a positive control had the highest activity, whereas the apu-apu sample which had the highest antioxidant activity was the ethanol-water fraction, and the lowest was the n-hexane fraction. The conclusion of this study is apu-apu herbal ethanol extract and its fractions have antioxidant activity, from high to low sequentially: ethanol-water fraction, ethyl acetate fraction, apu-apu herbaceous fraction, n- fraction hexane.

Keywords

DPPH,apu-apu, extract, fraction

Full Text:

PDF

References

Badarinath, A., Rao, K., Chetty, C. S., Ramkanth, S., Rajan, T,, & Gnanaprakash, K. 2010. A Review on In-vitro Antioxidant Methods: Comparisons, Correlations, and Considerations. International Journal of PharmTech Research. 1276-1285.

Depkes RI. 2000. Parameter Standar Umum Ekstrak Tumbuhan Obat (Edisi 1). Jakarta: Direktorat Jenderal Pengawasan Obat dan Makanan, Direktorat Pengawasan Obat Tradisional.

Edawati, Z. 2012. Uji Aktivitas Antioksidan Ekstrak Metanol Ascidia Didemnum sp. Dari Kepulauan Seribu dengan Metode 1,1-Difenil-2 Pikrilhidrazil (DPPH) dan Identifikasi Golongan Senyawa dari Fraksi Teraktif. Skripsi. FMIPA UI: Depok.

Halliwell, B. & Gutteridge, J. M. C. 2000. Free Radical in Biology and Medicine. Newyork: Oxford University Press.

Ito, N., M. Hirose, S. Fukishima, H. Tsada, T. Shirai, & M. Ttematsu. 1986. Studies on Antioxidants: Their Anticarcinogenic and Modifying, Effects on Chemichal Carcinogenesis. Food Chem. Toxico.l, (24):1099-1102.

Khan, M.A. et al. 2014. Pistia stratiotes L. (Araceae) : Phytochemistry, Use In Medicines, Phytoremediation, Biogas, and Management Options. Pak. J. Bot., 46(3): 851-860.

Khare, C.P. 2005. Encyclopedia of Indian medicinal plants. Berlin Heidelberg, Germany: Springer-Verlag.

Kumalaningsih, S., 2006. Antioksidan Alami. Surabaya: Trubus Agrisarana.

Kumar, H.K.S., M.B.V. Raju, S.C. Dinda, S. K. Sahu & M. Banerjee. 2011. Analgesic, anti-inflammatory and antipyretic activity of Pistia stratiotes L. Rasayan J. Chem., 4(3): 506-511.

Marinova, D., Ribanova, F., Atanassova, M. 2005. Total Phenolics and Total Flavonoids in Bulgarian Fruits and Vegetables. Journal of the University of Chemical Technology and Metallurgy, (40): 255-260.

Molyneux, P. 2004. The Use of Stable Free Radical Diphenylpicryl hydrazyl (DPPH) for estimating antioxidant activity. Songklanakarin J. Sci. Technol, 26(2): 211-219.

Pallavi, T., S. Arora, R. Gupta & P. Mali. 2011. Diuretic activity of Pistia stratiotes leaf extract in rats. Int. J. of Pharmacy, 2(3): 249-251.

Phongpaichit, S., et al. 2007. Biological Activities of Extracts From Endophytic Fungi Isolated From Garcinia Plant. FEMS Immunology & Medical Microbiology, 51(3), 517 – 525.

Yuhernita & Juniarti. (2011). Analisis Senyawa Metabolit Sekunder dari Ekstrak Metanol Daun Surian yang berpotensi Sebagai Antioksidan. Makara Sains, 15(1), 48 – 52.

DOI: 10.23917/pharmacon.v16i2.9148

Article Metrics

Abstract view(s): 814 time(s)
PDF: 997 time(s)

Refbacks

  • There are currently no refbacks.