Uji Aktivitas Antioksidan Ekstrak Etanol 70% Bunga Telang (Clitoria ternatea L) dari Daerah Sleman dengan Metode DPPH

Disa Andriani(1*), Lusia Murtisiwi(2)

(1) 2Departement of Natural Pharmaceutical Technology and Drug Synthesis, Pharmacy Undergraduate Study Program, SekolahTinggi Ilmu Kesehatan Nasional, Solo-Baki Kwarasan Street, Grogol, Sukoharjo, Central Java, 57552
(2) 2Departement of Natural Pharmaceutical Technology and Drug Synthesis, Pharmacy Undergraduate Study Program, SekolahTinggi Ilmu Kesehatan Nasional, Solo-Baki Kwarasan Street, Grogol, Sukoharjo, Central Java, 57552
(*) Corresponding Author

Abstract

Telang flower (Clitoria ternatea L.) contains phenolic compounds which can act as antioxidants by donating hydrogen so as to stabilize the lack of electrons in free radicals. This study aims to determine the antioxidant potential of telang flower ethanol extract by looking at the IC50 value. Telang flowers were extracted by maceration method using 70% ethanol solvent. The potential for antioxidants was determined by the DPPH (1,1-diphenyl-2-picrylhydrazyl) method with a comparison of vitamin C which had been shown to have the potential of free radicals which were very potent. The results showed the IC50 value of telang flower ethanol extract was 41.36 ± 1.191 μg / mL, based on that value the telang flower extract from the Sleman area was in the very potent category so that it could be developed as a source of antioxidants from natural ingredients.

Keywords

telang flower extract; antioxidant; DPPH; IC50

Full Text:

PDF

References

Andriani, Disa dan Murtisiwi, Lusia, 2018, Penetapan Kadar Fenolik Total Ekstrak Etanol Bunga Telang (Clitoria ternatea L.) dengan Spektrofotometri UV Vis, Cendekia Journal of Pharmacy, 2 (1): 32-38.

Departemen Kesehatan RI, 2008, Farmakope Herbal Indonesia Edisi I, Jakarta, 174.

Halliwell, B., Gutteridge, J. M., 1989, Free radicals in biology and medicine, 2nd ed, Oxford: Clarendon Press.

Huda, F. N., Noriham, A., Norrakiah, A. S., dan Babji, A. S., 2009, Antioxidant activity of plants methanolic extracts containing phenolic compounds, African Journal of Biotechnology, 8 (3), 484-489.

Kazuma, K., Noda, K., Suzuki, M., 2013, Flavonoid composition related to petal color in different lines of Clitoria ternatea, Phytochemistry, 64 (1133-1139).

Kähkönen, M.P., Hopia, A.I., Vuorela, H.J., Rauha, J.-P., Pihlaja, K., Kujala, T.S., dkk., 1999. Antioxidant activity of plant extracts containing phenolic compounds. Journal of agricultural and food chemistry, 47: 3954–3962.

Reynertson, K.A., 2007, Phytochemical Analysis of Bioactive Constituens from Edible Myrtaceae Fruit, The City University of New York, New York.

Rohman dan Riyanto, 2004, Aktivitas antioksidan ekstrak buah mengkudu (Morinda citrifolia, L), Agritech, 25(3): 131-136.

Vierkötter, A. dan Krutman, J., 2012, Environmental Influences on Skin Aging and Ethnic-Specific Manifestations, Dermatoendocrinol, 4 (3), 227-231.

Widhihastuti, E, 2011, Pengukuran Aktivitas Antioksidan dengan Metode DPPH serta Korelasinya dengan Kadar Fenolik Pada Lima Jenis Herba Bahan Obat Alam Indonesia, Skripsi, Universitas Muhammadiyah Surakarta, Surakarta.

Young, I.S., Woodside, J.V, 2001, Antioxidants in health and disease, J Clin Pathol, 54:76-86.

Article Metrics

Abstract view(s): 18844 time(s)
PDF: 18888 time(s)

Refbacks

  • There are currently no refbacks.