Deteksi Gen SHV pada Isolat Klinik Eschericia coli Penghasil Extended Spectrum Beta-Lactamases (ESBLs) dari Urin Pasien di RSUD Dr. Soetomo Surabaya

Yulianto Ade Prasetya(1*)

(1) Program Studi Teknologi Laboratorium Medik, STIKES RS Anwar Medika Sidoarjo
(*) Corresponding Author

Abstract

Esherichia coli ESBLs bertanggungjawab terhadap terjadinya wabah infeksi nosokomial, peningkatan morbiditas dan mortalitas, serta peningkatan biaya kesehatan. Tujuan penelitian ini yakni untuk mendeteksi keberadaan gen SHV pada isolat klinik E.coli penghasil ESBLs dari urine pasien yang merupakan koleksi Laboratorium Mikrobiologi Klinik RSUD Dr. Soetomo Surabaya pada bulan Januari - Februari 2014. Jenis penelitian yang digunakan adalah observasional deskriptif dengan pendekatan molekul genetik. Metode yang digunakan untuk deteksi gen SHV menggunakan PCR (Polymerase Chain
Reaction) kemudian dielektroforesis dan divisualisasikan pada gel agarose 1.5%. Hasil penelitian menunjukkan bahwa dari tiga puluh isolat, sebanyak dua belas isolat (40%) positif mengandung gen SHV.

Keywords

ESBLs, Eschericia coli, RSUD Dr. Soetomo Surabaya, SHV

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References

Adelyap, M.A., Harbart, S., Vernaz, N., Kearney, M.P., Scott, M.G., Elhajji, F.W.D., Aldiab M.A dan Mc Elnay, J.C. 2011. The impact of antibiotic use on the incidence and resistance pattern of extended-spectrum beta-lactamase-producing bacteria in primary and secondary healthcare

settings. British Journal of Clinical Pharmacology. DOI:10.1111/.1365-2125.

Bush, K. & Jacoby, G. A. 2010. Updated functional classification of β-lactamases. Antimicrob Agents

Chemother. 54: 969-976.

Colodner, R. 2013. Extended-Spectrum Beta-Lactamases: The End of Cephalosporins; http://www.ima.org.il/imaj/ar05may-13.pdf.

Ferreira, C.M., Ferreira, W. A., Almeida, N.O., Gomes, F., Naveca, and Barbosa, M.G. 2011. Extended-Spectrum Beta-Lactamase-Producing Bacteria Isolated From Hematologic Patients In Manaus, State Of Amazonas, Brazil. Brazilian Journal of Microbiology. 42: 1076-1084.

Foxman, B. 2010. The epidemiology of urinary tract infection. Nat Rev Urol 7(12): 653-660.

Garza-Ramos, U., Martinez-Romero, E., and Silvia-Sanchez, J. 2007. SHV-type Extended spectrum ß-lactamase (ESBL) are encoded in related plasmids from Enterobacteria clinical isolates from Mexico. Salud Publica Mex. 49(6): 415-421.

Jan, N., Sudhir, U., and Archana K. 2009. Plasmid profile analysis of multidrug resistant E.coli isolated from UTI patients of Nagpur City, India. Romanian Biotechnological Letters. 14 (5):4635-4640.

Kiratisin, P., Apisarnthanarak, A., Laesripa, C., and Saifon, P. 2008. Molecular characterization and epidemiology of extended-spectrum ß-lactamase producing Escherichia coli and Klebsiella pneumoniae isolates causing health care-associated infection in Thailand, where the CTX-M family is endemic. Antimicrob Agents Chemother.52:2818-2824.

Kuntaman, K., Santoso, S., Wahjono, H., Mertaningsih, N.M., Lestrasi, E.S., Farida, H.,Hapsari, R., Firmanti, S.C., Noorhamdani, A.S., Santosaningsih, D., Purwono, P.B., and

Kusumaningrum, D. 2011. The sensitivity pattern of extended spectrum beta lactamase-producing bacteria against six antibiotics that routinely used in clinical setting. J. Indon MedAssoc. 61(12): 482-486.

Paterson, D.L. 2010. Extended-Spectrum β-Lactamases: a Clinical Update; downloaded at http://www. ncbi.nlm. nih.gov/pmc/articles/PMC1265908/pdf/0016-05.

Pratiwi, S.T. 2008. Mikrobiologi Farmasi. Penerbit Erlangga. Jakarta.hlmn 151.

Severin, J.A., Mertaningsih, N.M., Kuntaman, K., Lestari,E.S., Purwanta, M., Toom, N.L.,Duerink, D.O., Hadi, U., Belkum, A., Verbrugh, H.A. and Goessens, W.H. 2010. Molecular characterization of extended-spectrum β-lactamases in clinical Escherichia coli and Klebsiella

pneuomniae isolates from Surabaya, Indonesia. J. Antimicrob Chemother. 65: 465-469.

Sharma, J., Sharma, M and Ray, P. 2010. Detection of TEM & SHV genes in Eschericia coli & Klebsiella pneuomoniae isolates in a tertiary care hospital from India. Indian J Med Res. 132:332-336.

Sorlozano, A., Guteirrez, J., Luna, J.D., Oteo, J., Liebena, J., Soto, M.J., and Piedrola, G. 2007.High presence of extended-spectrum β-lactamases and resistance to quinolones in clinical isolates of Escherichia coli. Microbiological Research. 162: 347-354.

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