Uji Aktivitas Wound Healing Ekstrak Etanol Buah Averrhoa bilimbiL. (Belimbing Wuluh) pada Mencit Putih Jantan (Mus musculus)

Rahmayati Rusnedy(1*), Mira Febrina(2), Cindy Patika Sari(3)

(1) STIFAR RIAU
(2) sekolah tinggi ilmu farmasi riau
(3) sekolah tinggi ilmu farmasi riau
(*) Corresponding Author

Abstract

Wound healing is a complex process, delays in wound healing increase the chances of infection, improper healing, and unpleasant scarring. Belimbing wuluh (Averrhoa bilimbi L.) fruit contains various active compounds that are beneficial to health and have the potential to be used as medicinal preparations for treating wounds. In this test, incisions were made on the skin of the mice's back area using a scalpel 1 cm long and 0.3 cm deep. Observation of the length of the incision was carried out every day for 12 days using a caliper. Significant wound healing activity was observed from administration of 10% concentration of ethanol extract of belimbing wuluh (Averrhoa bilimbi L.) fruit which contains chemical compounds such as flavonoids, saponins, tannins and triterpenoids as well as minerals, one of which is zinc and Vitamin C is also proven to help speed up the healing process of incisions with a percentage of wound healing on day 12, which is 100%, the same percentage value as the group of test animals given Povidone Iodine 10% (Betadine®) as a comparator drug. The presence of zinc (zinc) and vitamin C in the extract of star fruit (Averrhoa bilimbi L.), is predicted to also contribute to the wound healing process in this test.

Keywords

Ethanol extract of Averrhoa bilimbi L., Flavonoids, Saponins, Tannin, Triterpenoid, Zinc and Vitamin, Wound healing

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References

Agra L.C., Ferro J.N.S., Barbosa F.T., Barreto E., 2015. Triterpenes with healing activity: A systematic review. J. Dermatol. Treat. 26, pp.465–470.

Akiyama H, Fujii K, Yamasaki O, Oono T, Iwatsuki K., 2001. Antibacterial action of several tannins against Staphylococcus aureus. J Antimicrob Chemother, 48(4), pp.487–491.

Ansari T. M., N. Ikram, M. Najam-ul-Haq, I. Fayyaz, Q. Fayyaz, I. Ghafoor., 2004. Essentıal trace metal (zinc, manganese, copper and iron) levels ın plants of medicinal importance, Journal of Biological Sciences, 4, pp.95–99.

Anyakudo M. M. C. and Erinfolami A. B., 2015. Glycemic and wound healing effects of aqueous mesocarp extract of unripe Carica papaya (Linn) in diabetic and healthy rats, World Journal of Nutrition and Health, 3, pp.47–52.

Bakondi E, Bai P, Erdelyi K, Szabo C, Gergely P, Virag L., 2004, Cytoprotective effect of gallotannin in oxidatively stressed HaCaT keratinocytes: the role of poly(ADP-ribose) metabolism. Exp Dermatol. 13(3), pp.170–178.

Bhar R., S. K. Maiti, T. K. Goswami, R. C. Patra, A. K. Garg, and Chhabra A. K., 2003, Effect of dietary vitamin C and zinc supplementation on wound healing, immune response and growth performance in swine, Indian Journal of Animal Sciences, 736, pp.674–677.

Bigliardi P., L. Alsagoff, S.A.L., El-Kafrawi H.Y., Pyon J.K., Wa C.T.C., Villa M, A., 2017, Povidone iodinein wound healing: A review of current concepts and practices. Int J Surg. 44, pp 260-268.

Bhaskar B dan Shantaram M., 2013, Morphological and biochemical characteristics of Averrhoa fruits. International Journal of Pharmaceutical, Chemical and Biological Sciences, 3(3), pp.924-928.

Buzzini P, Arapitsas P, Goretti M., 2008, Antimicrobial and antiviral activity of hydrolysable tannins. Mini Rev Med Chem. 12, pp.1179–1187.

ChenY. , L. Tian, F. Yang, W. Tong, R. Jia, Y. Zou, L. Yin, L. Li, C. He, X. Liang, G. Ye, C. Lv, X. Song, Z. Yin., 2019, Tannic acid accelerates cutaneous wound healing in rats via activation of the ERK 1/2 signaling pathways. Adv. Wound Care, 8, pp.341-354.

Cukjati, D., Rebersek, S. and Miklavcic, D., 2001, A reliable method of determining wound healing rate. Med. Biol. Eng. Comput. 39, pp.263–271.

Ehrlich H. P. and T. K. Hunt, 1969, The effects of cortisone and anabolic steroids on the tensile strength of healing wounds, Annals of Surgery, 170(2), pp.203–206.

Getie M., M. T. Gebre, R. Reitz, and R. H. Neubert, 2002, Evaluation of the release profiles of flavonoids from topical formulations of the crude extract of the leaves of Dodonea viscosa (Sapindaceae), Pharmazie, 57. pp.320–322.

Gomathi K, Gopinath D, Ahmed MR, Jayakumar R., 2003, Quercetin incorporated collagen matrices for dermal wound healing processes in rat. Biomaterials, 24, pp.2767-2772.

Guo S., Dipietro L.A., 2010, Factors affecting wound healing. J Dent Res. 89(3), pp.219-29.

Gurtner G.C., Werner S., Barrandon Y., Longaker M.T., 2008, Wound repair and regeneration. Nature, 453, pp.314–321.

Han G, dan Ceilley, R., 2017, Chronic Wound Healing: A review of current management and treatments. Adv. Ther. 34, pp.599–610.

Hartini IGAA., 2012, Topical application of ethanol extract of starfruit leaves (Averrhoa bilimbi L.) increases fibroblasts in gingival wounds healing of white male rats, Indonesia Journal of Biomedical Science, 6(1), pp.35-39.

Haslam E., 1996, Natural polyphenols (vegetable tannins) as drugs: possible modes of action. J Nat Prod. 59(2), pp205–215.

Ibrahim N. I., Sok K.W., Isa N.M., Norazlina M., Kok-Yong C., Soelaiman I-N., dan Ahmad N. S., 2018, Wound healing properties of selected natural products. Int. J. Environ. Res. Public Health, 15, 2360.

Just MJ, Recio MC, Giner RM, Cuéllar MJ, Máñez S, Bilia AR, Ríos J-L., 1998, Anti-inflammatory activity of unusual lupane saponins from Bupleurum fruticescens. Planta medica, 64, pp.404-407.

Kim YS, Cho I-H, Jeong M-J, Jeong S-J, Nah SY, Cho Y-S, Kim SH, Go A, Kim SE, Kang SS, Moon CJ, Kim JC, Kim SH, Bae CS., 2011, Therapeutic effect of total ginseng saponin on skin wound healing, Journal of ginseng research, 35, pp.360-367.

Kimura Y, Sumiyoshi M, Kawahira K, Sakanaka M., 2006, Effects of ginseng saponins isolated from Red Ginseng roots on burn wound healing in mice. British journal of Pharmacology, 148, pp.860-870.

Kumar, K.A., Gousia, S., Anupama, M. dan Latha, J.N.L., 2013, A review on phytochemical constituents and biological assays of Averrhoa bilimbi. International Journal of PHarmacy and PHarmaceutical Science Research, 3(4), pp.136-139.

Kupeli, Akkol E., U. Koca, I. S Pesin, and D. Yilmazer, 2011, Evaluation of the wound healing potential of Achillea biebersteinii Afan. (Asteraceae) by in vivo excision and incision models, Evidence-Based Complementary and Alternative Medicine, 2011:474026.

Lansdown A. B. G., U. Mirastschijski, N. Stubbs, E. Scanlon, and M. S. Agren, 2007, Zinc in wound healing: theoretical, experi-mental, and clinical aspects, Wound Repair and Regeneration, 15(1) pp.2–16.

MacKay D. and A. L. Miller, 2003, Nutritional support for wound healing, Alternative Medicine Review, 8(4), pp.359–377.

Men SY, Huo QL, Shi L, Yan Y, Yang CC, Yu W, Liu BQ., 2020, Panax notoginseng saponins promotes cutaneous wound healing and suppresses scar formation in mice. Journal of Cossmetic Dermatology, 19(2), pp.529-534.

Metelmann, H.R., Brandner, J.M., Schumann, H., Brossd, F., Hoffmannd, M., Podmelle, F., 2012, Accelerating the aesthetic benefit of wound healing by triterpene. J. Craniomaxillofac. Surg., 40, pp.150–154.

Metelmann, H.R.., Brandner, J.M., Schumann, H., Bross, F., Fimmers, R., Böttger, K., Scheffler, A., Podmelle, F., 2015, Accelerated reepithelialization by triterpenes: Proof of concept in the healing of surgical skin lesions. Skin Pharmacol. Physiol, 28.

Meyer-Ingold W., 1993, Wound therapy: growth factors as agents to promote healing, Trends in Biotechnology, 11(9), pp.387–392.

Muhammad and Veronique, 2015. Isolation of Triterpenes from Propolis (Bee Glue), Bio-protocol. 5(20):e1630.

Natarajan V., N. Krithica, B., Madhan, P.K., Sehgal., 2013, Preparation and properties of tannic acid cross-linked collagen scaffold and its application in wound healing. J. Biomed. Mater. Res. B Appl. Biomater, 101, pp.560-567.

Navarro P, Giner RM, Recio MC, Máñez S, Cerdá-Nicolás M, Rı́os J-L., 2001, In vivo anti-inflammatory activity of saponins from Bupleurum rotundifolium L. Life Sciences, 68, pp.1199-1206.

Ninan N. , A. Forget, V.P. Shastri, N.H. Voelcker, A. Blencowe., 2016, Antibacterial and anti-inflammatory pH-responsive tannic acid-carboxylated agarose composite hydrogels for wound healing, ACS Appl. Mater. Interfaces, 8, pp.28511-28521.

Orwa, C., Mutua, A., Kindt, R., Jamnadass, R. dan Anthony, S. 2009. Averrhoa bilimbi L.. pp.1–5. Kenya: Agroforestree Database.

Patil AG, Koli SP, Patil DA., 2013, Pharmcognostical standardization and HPTLC fingerprint of Averrhoa bilimbi (L.) Fruits. Journal of Pharmacy Research, 6(2013), pp.145-150.

Sakar M. K. and M. Tanker., 1991, Phytochemical Analysis (Determination, Quantitative Analysis and Isolation), Ankara University, Faculty of Pharmacy, Ankara,Turkey.

Samah A.R. , B.M. NaserEldin , Z.A Aisha., 2016, In vivo antibacterial and wound healing activities of Acacia ehrenbergiana, Mediterr. J. Biosci. 1(4), pp.147–152.

Santana L. F., A. C. Inada, B. L. S. do Espirito., 2019, Nutraceutical potential of Carica papaya in metabolic syndrome, Nutrients, 11(7), pp.1608.

Sharma A, Suryamani K, Gaganjot K and Inderbir S., 2021, Medicinal plants and their components for wound healing applications, Future Journal of Pharmaceutical Sciences. 7:5.

Sharma S. P, Aithal K. S., K. K. Srinivasan, A. L. Udupa, V. Kumar, D. R. Kulkarni, 1990, Anti-inflammatory and wound healing activities of the crude alcoholic extracts and flavonoids of Vitex leucoxylon, Fitoterapia, 61, pp.263–265.

Shetty S., S. Udupa, and L. Udupa., 2008, Evaluation of antioxidant and wound healing effects of alcoholic and aqueous extract of Ocimum sanctum Linn in rats, Evidence-Based Complementary and Alternative Medicine, 5(1), pp.95–101.

Su X, Liu X, Wang S, Li B, Pan T, Liu D, Wang F, Diao Y, Li K., 2017, Wound-healing promoting eff ect of total tannins from Entada phaseoloides (L.) Merr. in rats. Burns. 43(4), pp.830-838.

Suntar I.P., U. Koca, H. Keles, and A. E. Kupeli, 2011a, Wound healing activity of Rubus sanctus Schreber (Rosaceae): preclinical study in animal models, Evidence-Based Complementary and Alternative Medicine. 2011: 816156.

Suntar I.P., Ufuk K., E.K Akkol, Demet Y., dan Murat A., 2011b. Assessment of Wound Healing Activity of the Aqueous Extracts of Colutea cilicica Boiss. & Bal. Fruits and Leaves. Hindawi Publishing Corporation Evidence-Based Complementary and Alternative Medicine. 2011:758191.

Teixeira D.S., Maria A.Z.F., Karen C., Sílvia D.O., Fernanda G.S,. 2019. The topical effect of chlorhexidine and povidone-iodine in the repair of oral wounds. A review. Stomatologija. Baltic Dental and Maxillofacial Journal, 21, pp 35-41.

Yan SW, Ramasamy R, Alitheen NBM, Rahmat A., 2013, A Comparative Assessment of Nutritional Composition, Total Phenolic, Total Flavonoid, Antioxidant Capacity, and Antioxidant Vitamins of Two Types of Malaysian Underutilized Fruits (Averrhoa bilimbi and Averrhoa carambola). International Journal of Food Properties, 16(6), pp.1231– 1244

DOI: 10.23917/pharmacon.v20i1.22677

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